Tag Archives: Pear psylla

Molecular Study of Pear Psylla Cacopsylla spp. (Psyllidae:  Hemiptera) in Middle and Southern Regions of Syria

Bassam Oudeh*(1)  Wajih Kassis (2) and Randa Abu-Tara (3)

(1). Mokhtaria Research Station, Agricultural Scientific Research Center at Homs, General Commission for Scientific Agricultural Research (GCSAR), Homs, Syria.
(2). Department of Plant Protection, Faculty of Agriculture, Damascus University, Damascus, Syria.
(3). Faculty of Science, Damascus University, Damascus, Syria.

(*Corresponding author: Dr. Bassam Oudeh. E-Mail: B_oudeh@hotmail.com).

Received: 01/02/ 2015                                     Accepted: 31/07/ 2015

Abstract

Molecular study of pear psylla Cacopsylla spp. (Psylldae: Hemiptera) was carried out in the biotechnology laboratory, Faculty of Agriculture, Damascus University during the growing season 2013/ 2014. Samples were collected from four governorates located in middle and southern regions of Syria: Homs (Mokhtaria Research Station and Al- Rastan), Hama (Tezeen), Damascus rural (Al-Zabadany) and Al-Sweida (Al-Sweida Research Center). The results showed that genetic differences of pear psylla by using ISSR with 19 primers, only 12 primers were succeeded in amplifying the male ́s DNA of pear psylla. The total number of bands was 70, only 64 bands were polymorphic, the percentage of polymorphism between males was (93.06%), males of pear psylla of Damascus rural and Al-Sweida were the most closer (86.12%), the cluster analysis separated the males of  Hama in an independent first group, and the second was separated into two sub clusters, males of Al-Rastan and Mokhtaria Research Station were included in the first sub clusters,  males of Damascus rural and Al-Sweida were included in the second. Only 15 primers were succeeded in amplifying the female ́s DNA of pear psylla. The total number of bands were 70, only 61 bands were polymorphic, the percentage of polymorphism between females was (74.49%), females of Al-Rastan and Hama were the most closer (77.22%), the cluster analysis separated the females of  Damascus rural in an independent first group, and the second group was separated into two sub clusters, females of Al-Sweida were included in the first sub clusters, and females of other regions were included in the second, as a conclusion, males and females of pear psylla were separated according to geographical distribution and species.

Key words: Pear psylla, Cacopsylla spp., Molecular study, ISSR , Syria

Full paper in Arabic:

 دراسة جزيئية لحشرات بسيلا الأجاص (Cacopsylla spp. (Psyllidae: Hemiptera في المنطقتين الوسطى والجنوبية من سورية

Repellent Effect of Kaolin Particle Film, (SURROUND) on Eggs Laying of Cacopsylla bidens (Šulc). (Psyllidae; Hemiptera)

MazenBufaur(¹), Vili Harizanova(²) & Nariman Al-Zugbi(¹)

(1). Scientific Agriculture Research Centre of Swieda.P.O. Box: 461, Swieda, Syria.
(2). Agricultural University, Plovdiv, Bulgaria.

(Corresponding author: Dr. Mazen Bufaur, Scientific Agriculture Research Centre of Swieda .P.O. Box: 461, Swieda, Syria. E-mail: mazenaudy@yahoo.com).

                 Received: 09 / 07 / 2014                 Accepted: 02 / 12 / 2014

Abstract:

Field experiments were conducted in 2012, 2013 on pear trees in the Scientific Agricultural Research Center of Sweada, Syria, to study the efficacy of kaolin-particle film Surround WP® (Engelhard Corp.) on lowering the number of eggs laid by the over-wintering females of pear psylla Cacopsylla bidens (Šulc,1907). Two treatments (A & B) were conducted and control was untreated. Treatment A with a single pre-blooming application and treatment B with two pre-blooming applications. In 2012, the efficacy of one application was 98.2% in treatment A and 97.4% in treatment B. However after the second application in the treatment B the efficacy reduced to 94.2% while in case single application (treatment A) the efficacy reduced to 85.65%. In 2013 the efficacy of kaolin-particle film in pre-blooming first application were 84.05%, 81.55% in treatments A and B respectively, and after  second application to B treatment, the recorded efficacy were 83.12%, 95.82% in treatments A and B respectively. The experiment was also conducted on the foliage of pear trees in the previous two years and the efficacy of Surround WP® was 90.82%, 89.15% in treatment B in 2012, 2013 respectively while in A were 69.74%,73.84% respectively. In a laboratory experiment with treated and untreated pear leaves adult females spent significantly less time on treated compared to untreated.

Key words: Repellent effect, Particle film technology, Kaolin, Over-wintering females, Pear psylla, Cacopsylla bidens (Šulc).

Full paper in Arabic:

التأثير الطارد لمركب الكلس الميكروني (Surround) على وضع البيض لحشرة بسيلا الأجاص