Tag Archives: ISSR

Molecular Study of Pear Psylla Cacopsylla spp. (Psyllidae:  Hemiptera) in Middle and Southern Regions of Syria

Bassam Oudeh*(1)  Wajih Kassis (2) and Randa Abu-Tara (3)

(1). Mokhtaria Research Station, Agricultural Scientific Research Center at Homs, General Commission for Scientific Agricultural Research (GCSAR), Homs, Syria.
(2). Department of Plant Protection, Faculty of Agriculture, Damascus University, Damascus, Syria.
(3). Faculty of Science, Damascus University, Damascus, Syria.

(*Corresponding author: Dr. Bassam Oudeh. E-Mail: B_oudeh@hotmail.com).

Received: 01/02/ 2015                                     Accepted: 31/07/ 2015

Abstract

Molecular study of pear psylla Cacopsylla spp. (Psylldae: Hemiptera) was carried out in the biotechnology laboratory, Faculty of Agriculture, Damascus University during the growing season 2013/ 2014. Samples were collected from four governorates located in middle and southern regions of Syria: Homs (Mokhtaria Research Station and Al- Rastan), Hama (Tezeen), Damascus rural (Al-Zabadany) and Al-Sweida (Al-Sweida Research Center). The results showed that genetic differences of pear psylla by using ISSR with 19 primers, only 12 primers were succeeded in amplifying the male ́s DNA of pear psylla. The total number of bands was 70, only 64 bands were polymorphic, the percentage of polymorphism between males was (93.06%), males of pear psylla of Damascus rural and Al-Sweida were the most closer (86.12%), the cluster analysis separated the males of  Hama in an independent first group, and the second was separated into two sub clusters, males of Al-Rastan and Mokhtaria Research Station were included in the first sub clusters,  males of Damascus rural and Al-Sweida were included in the second. Only 15 primers were succeeded in amplifying the female ́s DNA of pear psylla. The total number of bands were 70, only 61 bands were polymorphic, the percentage of polymorphism between females was (74.49%), females of Al-Rastan and Hama were the most closer (77.22%), the cluster analysis separated the females of  Damascus rural in an independent first group, and the second group was separated into two sub clusters, females of Al-Sweida were included in the first sub clusters, and females of other regions were included in the second, as a conclusion, males and females of pear psylla were separated according to geographical distribution and species.

Key words: Pear psylla, Cacopsylla spp., Molecular study, ISSR , Syria

Full paper in Arabic:

 دراسة جزيئية لحشرات بسيلا الأجاص (Cacopsylla spp. (Psyllidae: Hemiptera في المنطقتين الوسطى والجنوبية من سورية

Evaluation of Some Atriplex Accessions under Salt Stress Conditions and their Molecular Characterization Using ISSR Technique

Basem Alsamman*(1) Yousef Wjhani(1) Marwan Cheikh-Al basatneh(2) and Wael Yousef(1)

(1). Biotechnology Department, General Commission for Scientific Agricultural Research (GCSAR), Damascus, Syria.
(2). Damascus University, Faculty of Sciences, Ecology Department.

(*Corresponding author: Dr. Basem Alsamman, Biotechnology Department, (GCSAR), Damascus, Syria. E-mail:  basemhsam1@yahoo.com).

Received: 08/08/ 2014                                                 Accepted: 09/03/ 2015

Abstract

The study was carried out at Saalow Research Station, in Der Al Zur, General Commission for Scientific Agricultural Research (GCSAR), Der Al Zur, Syria, during the agricultural seasons 2008/2009- 2009/2010, 2010/2011 to evaluate the performance of four accessions related to the genus Atriplex, i.e. A. leucoclada, A. canescens, A. halimus1 (from Syria) and A. halimus2 (from Tunisia) under soil salinity stress conditions depending on a number of morphological indicators. ISSR technique (Inter Simple Sequence Repeats) was used for molecular characterization using 10 primers. The results showed that the studied accessions varied in their response for salinity stress conditions, the average of plant height in Atriplex halimus2 (203.3 cm) was significantly higher than the other accessions, A. halimus1, A. leucoclada and A. canescens (177.8, 72 and 64.3 cm), respectively. A. halimus1 and A. halimus2 revealed the highest tolerance to soil salinity (EC20 dSm-1) followed by A. leucoclada and lastly A. canescens. Results showed vast genetic variation among the studied accessions, all primers proved their effectiveness in showing polymorphism between the studied accessions; primers gave 132 bands with a polymorphic percentage 98%. The number of bands of each primer varied from minimum 7 bands for the primer (ISSR-17899b) to maximum 22 bands in the primer (ISSR-862) with average of 13.2 bands of each primer.

Key words: Atriplex, Soil salinity, Molecular characterization, ISSR.

Full paper in Arabic : تقييم بعض مدخلات الرغل تحت ظروف الإجهاد الملحي وتوصيفها جزيئياً باستخدام تقنية ISSR