Tag Archives: Molecular characterization

Molecular Characterization and Genetic Diversity in Prunus ursina KY Genotypes in the Syrian Coast

Wafaa Choumane (1) Haytham Ismael(1) Safaa Sabbouh*(2) Mazen Rajab(3) and Ammar Amran(3)

(1). Faculty of Agriculture, Tishreen University, Lattakia, Syria.

(2). Administration of Horticulture, General Commission for Scientific Agriculture Research (GCSAR), Damascus, Syria.

(3). Department of Biotechnology, Latakia Research Center, GCSAR, Damascus, Syria.

Received: 16/06/2019                                Accepted: 02/08/2019

Abstract

The target of this research was the molecular characterization and the estimation of genetic diversity of trees belonging to P. ursina in the Syrian coastal area. This study was conducted during the period started from 2016 to 2018. 35 trees (genotypes) were collected from (6) sites (Jabla, Kasab, Drekesh, Kab, Brmant AL mshaeh and Msiaf) and were used for DNA analysis by 17 SSR primer pairs. The results showed that out of 17 primer pairs, only 6 were polymorphic and produced 14 different alleles. Number of alleles ranged from (2) to (4) with a mean of 2.3 alleles/primer pair. The genetic diversity was estimated for each locus and ranged from 0.37 (TPScp9F/ TPScp9R) to 0.51 (BPPCT038F/BPPCT038R) and (CPDCT014/AY862451). Dendrogram based on genetic distance results was established and two groups were obtained. The first group included samples from Brmant AL mshaeh, Kasab and Jabla, while the second group had samples from Msiaf, Kab and Drekesh. Samples from Drekesh were the most distant. No variation was detected between samples from the same site, but found among the different sites. Level of genetic diversity in P. ursina was low in the sites investigated, therefore there is a need to continue collecting more samples from other sites to detect the genetic diversity of p. ursina and keep them in gene bank gene bank in order to use them in future genetic improvement programs.

Key words: P. ursina, Molecular characterization, Geneticdiversity, Syrian coast.

Full Paper in Arabic: PDF

Evaluation of Some Atriplex Accessions under Salt Stress Conditions and their Molecular Characterization Using ISSR Technique

Basem Alsamman*(1) Yousef Wjhani(1) Marwan Cheikh-Al basatneh(2) and Wael Yousef(1)

(1). Biotechnology Department, General Commission for Scientific Agricultural Research (GCSAR), Damascus, Syria.
(2). Damascus University, Faculty of Sciences, Ecology Department.

(*Corresponding author: Dr. Basem Alsamman, Biotechnology Department, (GCSAR), Damascus, Syria. E-mail:  basemhsam1@yahoo.com).

Received: 08/08/ 2014                                                 Accepted: 09/03/ 2015

Abstract

The study was carried out at Saalow Research Station, in Der Al Zur, General Commission for Scientific Agricultural Research (GCSAR), Der Al Zur, Syria, during the agricultural seasons 2008/2009- 2009/2010, 2010/2011 to evaluate the performance of four accessions related to the genus Atriplex, i.e. A. leucoclada, A. canescens, A. halimus1 (from Syria) and A. halimus2 (from Tunisia) under soil salinity stress conditions depending on a number of morphological indicators. ISSR technique (Inter Simple Sequence Repeats) was used for molecular characterization using 10 primers. The results showed that the studied accessions varied in their response for salinity stress conditions, the average of plant height in Atriplex halimus2 (203.3 cm) was significantly higher than the other accessions, A. halimus1, A. leucoclada and A. canescens (177.8, 72 and 64.3 cm), respectively. A. halimus1 and A. halimus2 revealed the highest tolerance to soil salinity (EC20 dSm-1) followed by A. leucoclada and lastly A. canescens. Results showed vast genetic variation among the studied accessions, all primers proved their effectiveness in showing polymorphism between the studied accessions; primers gave 132 bands with a polymorphic percentage 98%. The number of bands of each primer varied from minimum 7 bands for the primer (ISSR-17899b) to maximum 22 bands in the primer (ISSR-862) with average of 13.2 bands of each primer.

Key words: Atriplex, Soil salinity, Molecular characterization, ISSR.

Full paper in Arabic : تقييم بعض مدخلات الرغل تحت ظروف الإجهاد الملحي وتوصيفها جزيئياً باستخدام تقنية ISSR