Study of Genetic Diversity and Correlation of some Important Traits of Barley (Hordeum vulgare L.( Genotypes Using Diversity Array Technology Markers (DArT)

Abdullatef Ahmad Al-Assaf*(1) Mohammad Shafik Hakim(1) Mohammad Fadi Basmaji(2) and Stefania Grando(3)

(1). Department of Field Crops, Faculty of Agriculture, University of Aleppo, Aleppo, Syria.

(2). Aleppo Research Center, General Commission for Scientific Agricultural Research (GCSAR), Damascus, Syria.

(3). International Crops Research Institute for Semi-Arid Tropics (ICRISAT), Hyderabad, India.

(*Corresponding author: Eng. Abdullatef Ahmad Al-Assaf. E-Mail: a.alassaf74@gmail.com).

Received: 13/11/2018                                Accepted: 02/12/2018

Abstract

The research was conducted in corporation between Faculty of Agriculture, Aleppo University and General Commission for Scientific Agricultural Research (GCSAR) and International Center for Agricultural Researches in Dry Area ICARDA at three locations: Tal Hadya, Homeimeh and Breda during two seasons 2008/2009 and 2099/2010. RCBD was used with three replications. The study aimed to estimate the genetic diversity in barley using 43 collected genotypes from seven different geographical regions in the world to be used in the breeding programs. In addition, to study the association between markers with grain yield and plant height and some root traits in barley seedlings under controlling conditions to determine assisted markers for selection this trait in dry areas. 70 DArT markers (Diversity Array Technology) were used in the DNA analysis, and 139 alleles were detected in the whole collection. The number of alleles detected per locus varied from zero to 2, with an average of 1.99 alleles per locus. The genetic diversity GD values ranged between zero and 0.5, with an average of 0.366, while the Polymorphism Information Content PIC values ranged between zero and 0.488 with average of 0.358. The high level of genetic diversity and the absence of genetic similarity among genotypes indicated high genetic variability levels within barley collection. The results indicated that the collection regions had high genetic diversity of barley genotypes. Moreover, there is a positive association between molecular markers and barley traits, as 27 markers were associated with the traits in whole barley genome.

Key words: Barley, Genetic diversity, Molecular markers, DArT, Association analysis.

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Genetic Diversity of Some Wheat (Triticum durum) Genotypes Using SSR Technique

Reham Abo Al-Kanj*(1) Ghinwa Lababidi(1) and Naim Al-Husien(2)

(1). Department of Biotechnology, Faculty of Technical Engineering, University of Aleppo, Aleppo, Syria.

(2). Aleppo Research Center, General Commission of Scientific Agricultural Research Center (GCSAR), Damascus, Syria.

(*Corresponding author: Reham Abo Al-Kang. E-Mail: perfume-1990@hotmail.com).

Received: 02/05/2018                                Accepted: 01/10/2018

Abstract

The understanding of wheat genetic variation is considered the basis for successful plant breeding programs.the use of molecular markers in genetic diversity assessment studies and in plant breeding programs has become critical. Therefore, the research aimed to studying the genetic diversity of durum wheat genotypes, it was carried out at the Biotechnology Laboratory of the Aleppo Research Center during 2016 – 2017. The DNA was extracted from all genotypes by using CTAB method, and determined its quality, purity, and quantity.  SSR technology is used to study the genetic diversity with 13 primers and the dendrogram was drew using UPGMA method according to Jaccard coefficient. The results showed there were 160  bands ranged between 100-2000 bp. PIC value was between 0.14-0.37, So the wms70، wms666، wms642، wms408 primers showed the best results. Cluster analysis showed that genotypes distributed in two main clusters. The similarity ranging from 0.21 – 0.9. This confirms the efficacy of SSR markers in detecting a wide range of genetic variation and its ability to identify genotypes.

Keywords: Durum wheat, Genetic diversity, Molecular markers, SSR.

Full paper in Arabic: PDF