Reham Abo Al-Kanj*(1) Ghinwa Lababidi(1) and Naim Al-Husien(2)
(1). Department of Biotechnology, Faculty of Technical Engineering, University of Aleppo, Aleppo, Syria.
(2). Aleppo Research Center, General Commission of Scientific Agricultural Research Center (GCSAR), Damascus, Syria.
(*Corresponding author: Reham Abo Al-Kang. E-Mail: perfume-1990@hotmail.com).
Received: 02/05/2018 Accepted: 01/10/2018
Abstract
The understanding of wheat genetic variation is considered the basis for successful plant breeding programs.the use of molecular markers in genetic diversity assessment studies and in plant breeding programs has become critical. Therefore, the research aimed to studying the genetic diversity of durum wheat genotypes, it was carried out at the Biotechnology Laboratory of the Aleppo Research Center during 2016 – 2017. The DNA was extracted from all genotypes by using CTAB method, and determined its quality, purity, and quantity. SSR technology is used to study the genetic diversity with 13 primers and the dendrogram was drew using UPGMA method according to Jaccard coefficient. The results showed there were 160 bands ranged between 100-2000 bp. PIC value was between 0.14-0.37, So the wms70، wms666، wms642، wms408 primers showed the best results. Cluster analysis showed that genotypes distributed in two main clusters. The similarity ranging from 0.21 – 0.9. This confirms the efficacy of SSR markers in detecting a wide range of genetic variation and its ability to identify genotypes.
Keywords: Durum wheat, Genetic diversity, Molecular markers, SSR.
Full paper in Arabic: PDF