Tag Archives: SSR

Genetic Diversity of Some Wheat (Triticum durum) Genotypes Using SSR Technique

Reham Abo Al-Kanj*(1) Ghinwa Lababidi(1) and Naim Al-Husien(2)

(1). Department of Biotechnology, Faculty of Technical Engineering, University of Aleppo, Aleppo, Syria.

(2). Aleppo Research Center, General Commission of Scientific Agricultural Research Center (GCSAR), Damascus, Syria.

(*Corresponding author: Reham Abo Al-Kang. E-Mail: perfume-1990@hotmail.com).

Received: 02/05/2018                                Accepted: 01/10/2018

Abstract

The understanding of wheat genetic variation is considered the basis for successful plant breeding programs.the use of molecular markers in genetic diversity assessment studies and in plant breeding programs has become critical. Therefore, the research aimed to studying the genetic diversity of durum wheat genotypes, it was carried out at the Biotechnology Laboratory of the Aleppo Research Center during 2016 – 2017. The DNA was extracted from all genotypes by using CTAB method, and determined its quality, purity, and quantity.  SSR technology is used to study the genetic diversity with 13 primers and the dendrogram was drew using UPGMA method according to Jaccard coefficient. The results showed there were 160  bands ranged between 100-2000 bp. PIC value was between 0.14-0.37, So the wms70، wms666، wms642، wms408 primers showed the best results. Cluster analysis showed that genotypes distributed in two main clusters. The similarity ranging from 0.21 – 0.9. This confirms the efficacy of SSR markers in detecting a wide range of genetic variation and its ability to identify genotypes.

Keywords: Durum wheat, Genetic diversity, Molecular markers, SSR.

Full paper in Arabic: PDF

Identification of Some Local Apple Cultivars and Genotypes Using SSR Markers

Bayan Muzher*(1) and Ola Al-Halabi(1)

(1). Biotechnology Division, Apple Center Department, General Commission for Scientific Agriculture Research (GCSAR), Damascus, Syria.

(*Corresponding author: Dr. Bayan Muzher. E-mail: bmuzher@hotmail.com).

Received: 24/08/2016                           Accepted: 27/09/2016

Abstract

The current research was carried out during 2012-2013 to identify and screening some of local apple cultivars and genotypes, which distributed in different environmental regions using SSR markers. Fifteen local apple cultivars and genotypes were collected, in addition to two commercial cultivars; Golden delicious and Royal gala and three seedling genotypes. Genetic analysis were achieved using 16 SSR primer pairs, 15 of them were able to detect the polymorphism in the studied genotypes. The total number of polymorphic alleles was 40, with polymorphism percentage of 97.56%. The number of alleles ranged between 1- 4 alleles, with an average 2.56 alleles per locus. Genetic similarity ranged from 13 to 100%. The Cluster analysis divided the studied genotypes into two clusters, the first cluster included Golden delicious with Royal gala and the two seedling genotypes A and B in addition to most of genotypes collected from the coastal region, and Skarji cultivar, while the second cluster included C seedling genotype besides the local apple cultivars Sukari1, Sukari2, and Shmamti, also four local apple genotypes. Expected heterozygosity (He) was 0.495, while observed heterozygosity (Ho) was 0.285. Consequently, SSR markers were able to detect the genetic variability among studied genotypes that belong to local apple cultivars, which lead to reduce the time and efforts for determining their genetic identity.

Key words: Apple, Local cultivars, SSR, Genetic similarity.

Full paper in Arabic: PDF