Bayan Muzher*(1) and Ola Al-Halabi(1)
(1). Biotechnology Division, Apple Center Department, General Commission for Scientific Agriculture Research (GCSAR), Damascus, Syria.
(*Corresponding author: Dr. Bayan Muzher. E-mail: bmuzher@hotmail.com).
Received: 24/08/2016 Accepted: 27/09/2016
Abstract
The current research was carried out during 2012-2013 to identify and screening some of local apple cultivars and genotypes, which distributed in different environmental regions using SSR markers. Fifteen local apple cultivars and genotypes were collected, in addition to two commercial cultivars; Golden delicious and Royal gala and three seedling genotypes. Genetic analysis were achieved using 16 SSR primer pairs, 15 of them were able to detect the polymorphism in the studied genotypes. The total number of polymorphic alleles was 40, with polymorphism percentage of 97.56%. The number of alleles ranged between 1- 4 alleles, with an average 2.56 alleles per locus. Genetic similarity ranged from 13 to 100%. The Cluster analysis divided the studied genotypes into two clusters, the first cluster included Golden delicious with Royal gala and the two seedling genotypes A and B in addition to most of genotypes collected from the coastal region, and Skarji cultivar, while the second cluster included C seedling genotype besides the local apple cultivars Sukari1, Sukari2, and Shmamti, also four local apple genotypes. Expected heterozygosity (He) was 0.495, while observed heterozygosity (Ho) was 0.285. Consequently, SSR markers were able to detect the genetic variability among studied genotypes that belong to local apple cultivars, which lead to reduce the time and efforts for determining their genetic identity.
Key words: Apple, Local cultivars, SSR, Genetic similarity.
Full paper in Arabic: PDF