Study of Genetic Diversity and Correlation of some Important Traits of Barley (Hordeum vulgare L.( Genotypes Using Diversity Array Technology Markers (DArT)

Abdullatef Ahmad Al-Assaf*(1) Mohammad Shafik Hakim(1) Mohammad Fadi Basmaji(2) and Stefania Grando(3)

(1). Department of Field Crops, Faculty of Agriculture, University of Aleppo, Aleppo, Syria.

(2). Aleppo Research Center, General Commission for Scientific Agricultural Research (GCSAR), Damascus, Syria.

(3). International Crops Research Institute for Semi-Arid Tropics (ICRISAT), Hyderabad, India.

(*Corresponding author: Eng. Abdullatef Ahmad Al-Assaf. E-Mail:

Received: 13/11/2018                                Accepted: 02/12/2018


The research was conducted in corporation between Faculty of Agriculture, Aleppo University and General Commission for Scientific Agricultural Research (GCSAR) and International Center for Agricultural Researches in Dry Area ICARDA at three locations: Tal Hadya, Homeimeh and Breda during two seasons 2008/2009 and 2099/2010. RCBD was used with three replications. The study aimed to estimate the genetic diversity in barley using 43 collected genotypes from seven different geographical regions in the world to be used in the breeding programs. In addition, to study the association between markers with grain yield and plant height and some root traits in barley seedlings under controlling conditions to determine assisted markers for selection this trait in dry areas. 70 DArT markers (Diversity Array Technology) were used in the DNA analysis, and 139 alleles were detected in the whole collection. The number of alleles detected per locus varied from zero to 2, with an average of 1.99 alleles per locus. The genetic diversity GD values ranged between zero and 0.5, with an average of 0.366, while the Polymorphism Information Content PIC values ranged between zero and 0.488 with average of 0.358. The high level of genetic diversity and the absence of genetic similarity among genotypes indicated high genetic variability levels within barley collection. The results indicated that the collection regions had high genetic diversity of barley genotypes. Moreover, there is a positive association between molecular markers and barley traits, as 27 markers were associated with the traits in whole barley genome.

Key words: Barley, Genetic diversity, Molecular markers, DArT, Association analysis.

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