Tag Archives: Morphological characterization

The Phenotypic Characterization of Some Damask Rose (Rosa ‎damascena Mill.) Genotypes Distributed in Latakia ‎Government

Tharwat Saleem Redwan*(1) Hafez Mohammed Mahfouz(1) and Mazen Ali Nassour(2)

(1). Scientific Agricultural Research Center of Lattakia, General Commission for Scientific

Agricultural Research (GCSAR), Damascus, Syria.

(2). Department of Horticulture, Faculty of Agriculture, Tishreen University, Lattakia, Syria.

(*Corresponding author: Eng. Tharwat Saleem  Redwan. E-Mail: tharwat.redwan@yahoo.com) .

Received: 12/12/2018                               Accepted: 08/04/2019

Abstract

This research aimed to study the morphological characterization and to determine the  variability among twenty Rosa damascene genotypes, which grown in different areas of  Latakia and  compare them with two genotypes from Marah location at the countryside of Damascus to  take advantage of high quality characteristics  in order to be improved in the future, and  to enhance its cultivation. The results of morphological characterization  of forty traits showed a clear difference between some studied genotypes. The highest differences were related to flowers and vegetative characters compared with fruits and seeds characters.  A dendrogram based on the morphological data showed clear separation between studied genotypes, and reached (85%) for flowers characters and reached (60%) for vegetative characters, while it attained (47%)  for  fruits and seeds characters. The study showed that there were a distinct differences for some genotypes which can be used in breeding and genetic improvement programs in the future.
 Key words: Genotypes,  Rosa damascena,  Morphological characterization, Syria.
Full paper in Arabic: PDF

Methods for Maintaining Insect Cells Sf9 and its Morphological Characterization

Manal Saleh*(1)

(1). General Organization of Biotechnology, Damascus, Syria.

(*Corresponding author: Eng. Manal Saleh. E-Mail: manalcapno@gmail.com).

Received: 04/10/2016                                 Accepted: 18/11/2016

Abstract

The insect cell lines have a great importance in scientific research such as: Physiological and biological studies, producing biopesticides and vaccines, biotechnology and toxicology. The insect cell line Sf9, is derived from an immature pupal ovarian tissue of Spodoptera frugiperda. These cells were cultured, sub-cultured (more than 23 subculture) and cryopreserved. Morphological characterization of Sf9 cells were performed by using three staining methods i.e. Trypan blue, Wright, and Giemsa. Three different shapes of Sf9 cells were observed and they were: Spherical cells (large and small ones); fibroblast-like cells, and phagocytic cells. Two different populations of cells were identified in Sf9 cell cultures according to nuclear number: Mononucleated and polynucleated to ensure cells surviving.

Key word: Sf9 insect cell lines, Spodoptera frugiperda, Subculture, Cryopreservation, Morphological characterization.

Full paper in Arabic: PDF