Manal Saleh^*(1)

(1). General Organization of Biotechnology, Damascus, Syria.

(*Corresponding author: Eng. Manal Saleh. E-Mail: manalcapno@gmail.com).

Received: 04/10/2016                                 Accepted: 18/11/2016

Abstract

The insect cell lines have a great importance in scientific research such as: Physiological and biological studies, producing biopesticides and vaccines, biotechnology and toxicology. The insect cell line Sf9, is derived from an immature pupal ovarian tissue of Spodoptera frugiperda. These cells were cultured, sub-cultured (more than 23 subculture) and cryopreserved. Morphological characterization of Sf9 cells were performed by using three staining methods i.e. Trypan blue, Wright, and Giemsa. Three different shapes of Sf9 cells were observed and they were: Spherical cells (large and small ones); fibroblast-like cells, and phagocytic cells. Two different populations of cells were identified in Sf9 cell cultures according to nuclear number: Mononucleated and polynucleated to ensure cells surviving.

Key word: Sf9 insect cell lines, Spodoptera frugiperda, Subculture, Cryopreservation, Morphological characterization.

Full paper in Arabic: PDF