In Vitro Micropropagation of Seedlings of Ceratonia siliqua L. ‎with Micro-Cuttings

Fadi Kazngi*(1) Talal Amin(1) and Hafez Mahfoud(2)

(1). Environment and Forestry Department, Faculty of Agriculture, Tishreen University, Latakia, Syria.

(2). Biotechnology Division, General Commission for Scientific Agricultural Research (GCSAR), Damascus, Syria.

(*Corresponding author: Eng. Fadi Kazngi. E-Mail:

Received: 30/07/2019                               Accepted: 24/09/2019


This study was carried out during 2018 and 2019 seasons at the Green House Nursery using S1 genotype of Ceratonia Siliqua L. which is grown at the site of Snobar Jablah in Lattakia Governorate to determine the best hormonal balance to multiply the vegetative growth and rooting the micro- cutting of the seedling and thus the possibility of multiplying the desired genotypes of mature carob plants to produce vegetative strains for re-infiltration in its degraded areas. This study found a successful and detailed In Vitro propagation system for rapid micropropagation of carob. 10% Sodium Hypochlorite for 20 minutes gave the best efficiency for surface sterilization of vegetative growth. Concentration of 0.5 mg/L of Gibberellin with BAP at a concentration of 0.5 mg/L gave the best average of shoots length (8.47 cm), while the seedling multiplying became better when using the BAP hormone at a concentration of 0.5 mg/L with 0.1 mg/L of AIB. The best concentration of rooting hormone AIB was 2 mg/L which achieved the best percentage of rooting (71.67%), and the best mean number of roots(5.43) , while the concentration of 1 mg/L achieved the best mean length of roots (4.25 cm).

Key words: Carob, Micropropagation, Benzyl Amino Purine BAP, Indol Butyric Acid AIB.

Full paper in Arabic: PDF

Micropropagation of Date Palm (Phoenix dactylifera L.) Khadhrawy cv.

Wathiq Abdulmajeed*(1) Zahra Al- Hattab(1) Mahmood Al- Ani(1) Shuker Ebraheem(1) and Jabbar Jabr(1)

(1). Department of Genetic Engineering, Biotechnology Center, Ministry of Science and Technology, Baghdad, Iraq.

(*Corresponding author: Dr. Wathiq Abdulmajeed.

Received: 06/04/2019                                Accepted: 03/08/2019


This research was conducted at the Tissue Culture Laboratory, Department of Genetic Engineering, Directorate of Agriculture Research, Ministry of Science and Technology, Iraq, in 2017. The main objective was to establish the best method for In Vitro micropropagation of Khadhrawy economical Iraqi date palm cultivar. Shoot tips, lateral buds and leaf primordial were detached from the offshoots and surface was sterilized with 1% sodium hypochlorite with Tween 20. Explants were cultured on modified MS medium supplemented with 10 mg.l-1 of NAA, 10 mg.l-1 2,4-D, 10 mg.l-1 NAA+ 2 mg.l-1 2,4-D or 10 mg.l-1 2,4-D + 2 mg.l-1  NAA for callus initiation. Induced calli were transferred to regeneration medium. Regenerated shoots were rooted on medium supplemented with 1 mg.L-1 IBA. The results showed that the highest quality of calli was induced on medium supplemented with 10 mg.l-1 2,4-D + 2 mg.l1  NAA which was significantly different from the other treatments. Moreover, the shoot tips were the best explants to induce calli from Khadrawy cultivar compared with leaf primordial and lateral buds. The interaction analysis showed that the highest quantity of calli was produced from shoot tips cultured on medium supplemented with 10 mg.l-1  2,4-D + 2 mg.l-1  NAA which was highly significant from  all other combinations, while the lowest quantity of calli was produced from lateral buds cultured on medium supplemented with 10 mg.l-1 NAA. The average number of plants regenerated from 0.5 g calli was 30 after 2 months. The rooted shoots were successfully acclimatized and transferred to the nursery.

Keywords: Date palm, Khadhrawy c.v., Micropropagation, 2,4-D, NAA

Full paper in English: PDF