Fadi Kazngi*(1) Talal Amin(1) and Hafez Mahfoud(2)
(1). Environment and Forestry Department, Faculty of Agriculture, Tishreen University, Latakia, Syria.
(2). Biotechnology Division, General Commission for Scientific Agricultural Research (GCSAR), Damascus, Syria.
(*Corresponding author: Eng. Fadi Kazngi. E-Mail: fadikazngi79@yahoo.com).
Received: 30/07/2019 Accepted: 24/09/2019
Abstract
This study was carried out during 2018 and 2019 seasons at the Green House Nursery using S1 genotype of Ceratonia Siliqua L. which is grown at the site of Snobar Jablah in Lattakia Governorate to determine the best hormonal balance to multiply the vegetative growth and rooting the micro- cutting of the seedling and thus the possibility of multiplying the desired genotypes of mature carob plants to produce vegetative strains for re-infiltration in its degraded areas. This study found a successful and detailed In Vitro propagation system for rapid micropropagation of carob. 10% Sodium Hypochlorite for 20 minutes gave the best efficiency for surface sterilization of vegetative growth. Concentration of 0.5 mg/L of Gibberellin with BAP at a concentration of 0.5 mg/L gave the best average of shoots length (8.47 cm), while the seedling multiplying became better when using the BAP hormone at a concentration of 0.5 mg/L with 0.1 mg/L of AIB. The best concentration of rooting hormone AIB was 2 mg/L which achieved the best percentage of rooting (71.67%), and the best mean number of roots(5.43) , while the concentration of 1 mg/L achieved the best mean length of roots (4.25 cm).
Key words: Carob, Micropropagation, Benzyl Amino Purine BAP, Indol Butyric Acid AIB.
Full paper in Arabic: PDF