Assessment the Effect of Aqueous Extract of Peganum ‎harmala Seeds on Fertility of Male Mice

Samira Musa ELghul*(1) Nagia Musa Sasi(2) and  Ebtesam Mohammed Ahmed(3)

(1). Zoology Department, Faculty of Science, Tripoli University, Libya.

(2). Biotechnology Center, Tripoli, Libya.

 (3). Zoology Department, Collage of Science, Sirte University, Libya.

(*Corresponding author: Samira Musa ELghul. E. Mail:

Received: 22/03/2020                               Accepted: 18/05/2020


Peganum harmala is one of the medicinal plants, it is a widely used in a traditional medicine to treat several diseases, so the aim of this study was to evaluate of effect of aqueous extract of Peganum harmala seeds on fertility in male mice. Sixteen male mice were used in this study at age of 6 – 10 weeks, they were divided into two groups of 8 mice each, the control group was given distilled water by mouth, while another group was received aqueous extract of Peganum harmala seeds at a dose of (300 mg/kg) for three weeks. The results showed a significant decline in body and epididymis weight in the treated group comparing to the control group. There was no any effect of aqueous extract of Peganum harmala on sperm parameters and testicular tissue. Also no significant change was observed in percent of deformed embryos and dead embryos of females impregnated by males treated with the aqueous extract when compare to control.

Key words: Peganum harmala, Male mice, Testis, Fertility.

Full paper in English: PDF

Histological Study of Testicular Structure in Growing Syrian Buffalo Males

Nader Drbas*(1) and Solieman Salhab(2)

(1). Shatha Research Station, Agricultural Research Center in AL-Ghab, General Commission for Scientific Agricultural Research (GCSAR), Al Ghab, Syria.
(2). Animal Production Department, Faculty of Agriculture, Damascus University, Damascus, Syria.

(*Corresponding author: Eng: Nader Drbas. (GCSAR), Al Ghab, Syria).

Received: 05/07/ 2015                                     Accepted: 06/12/ 2015            


This study was conducted at Shatha Station of Syrian Buffalo Improvement, Hamah governorate, Al-Ghab province, Animal Wealth Research Administration, General Commission for Scientific Agricultural Research (GCSAR)/Syria, during 2011. Ten growing Syrian buffalo males aged 7 months were used. The body weight (BW), and testicular parenchyma sections had taken, and median diameters of somniferous tubules were measured monthly for 10 months, to determine the age at which spermatogenesis takes place, and when spermatozoa appears in the lumen of somniferous tubules (ST), as a predictable indicator of reaching puberty in the growing Syrian buffalo males. Results indicated that a gradual expansion in the diameter of (ST), also a development in Leydig cells, and appearance of Sertoli cells, with the advance in animal age. The average of (ST) was 172.7±2.8 µm in the age of 10 months, where the highest change in (ST) was recorded (P<0.05). At the age 16 months the average of (ST) was 250±6.1 µm, and spermatozoa in the lumen of somniferous tubules was noticed. Results showed positive significant correlations (p<0.01), between somniferous tubules and body weight (r= 0.983), also there was positive correlation between somniferous tubules and age of animal (r=0.978). It was concluded that puberty occurs in the growing Syrian buffalo males at approximately 16 months old, with an average in (BW) of 316 ± 14.1 kg.

Keywords: Syrian buffalo, Testis, Histological structure, Spermatogenesis, Sexual puberty.

Full paper in Arabic:دراسة نسيجية لبنية الخصى في ذكور الجاموس السوري النامية