Tag Archives: Gamma rays

TheAhmed Moursy*(1) and Mazen Ismail(1)(1). Soil   and   Water Research Department, Nuclear   Research   Center, Atomic   Energy   Authority, Abou-Zaabl, Egypt.(*Corresponding author: Ahmed Moursy. E-mail address ahmad1a2m3@yahoo.com).Received: 07/04/2019                                Accepted: 11/05/2019AbstractAn experiment was conducted at the Soils and Water Research Department, Nuclear Research Center, Atomic Energy Authority, Egypt, in the year 2018, to study the effect of gamma radiation at different doses (zero, 40, 80 and 160 Gy) on growth and yield of wheat fertilized with 0.0, 120 and 240 N kg ha-1 as urea fertilizer. The experiment was laid out in randomized complete block design with three replicates. Results revealed that, straw and grains yields were significantly fluctuated between the increase and decrease, furthermore, under 80 Gy gamma rays, the highest value was 15.677 kg plot-1, observed at rate of 240 N kg ha-1 in addition to 80 Gy. Grains increased compared to all treatments and control. Under gamma rays, the highest values of straw and grains were 9.974 kg plot-1 and 15.677 kg plot-1 observed rate of 240 kg ha-1 plus dose of 40 and 80 Gy radiation respectively. The data indicated that, N uptake by straw and grains, in non-irradiated treatments, were significantly increased with increasing the doses of nitrogen fertilizer levels 100 kg N fed-1 and gamma rays dose up to 80 Gy. Furthermore, the highest N uptake of 98.3 g plot-1 and 181.1 g plot-1 were observed at rate of 240 kg N ha-1 in addition to zero Gy, compared to control, whereas, under doses 80 Gy the highest values of N uptake were 120.1 g plot-1 and 195 g plot-1 which recorded with straw weight and grains yield, respectively.The Effect of Gamma Rays and Different levels of Nitrogen Fertilizer on the Productivity of Wheat (Triricum aestivum L.)

Ahmed Moursy*(1) and Mazen Ismail(1)

(1). Soil   and   Water Research Department, Nuclear   Research   Center, Atomic   Energy   Authority, Abou-Zaabl, Egypt.

(*Corresponding author: Ahmed Moursy. E-mail address ahmad1a2m3@yahoo.com).

Received: 07/04/2019                                Accepted: 11/05/2019

Abstract

An experiment was conducted at the Soils and Water Research Department, Nuclear Research Center, Atomic Energy Authority, Egypt, in the year 2018, to study the effect of gamma radiation at different doses (zero, 40, 80 and 160 Gy) on growth and yield of wheat fertilized with 0.0, 120 and 240 N kg ha-1 as urea fertilizer. The experiment was laid out in randomized complete block design with three replicates. Results revealed that, straw and grains yields were significantly fluctuated between the increase and decrease, furthermore, under 80 Gy gamma rays, the highest value was 15.677 kg plot-1, observed at rate of 240 N kg ha-1 in addition to 80 Gy. Grains increased compared to all treatments and control. Under gamma rays, the highest values of straw and grains were 9.974 kg plot-1 and 15.677 kg plot-1 observed rate of 240 kg ha-1 plus dose of 40 and 80 Gy radiation respectively. The data indicated that, N uptake by straw and grains, in non-irradiated treatments, were significantly increased with increasing the doses of nitrogen fertilizer levels 100 kg N fed-1 and gamma rays dose up to 80 Gy. Furthermore, the highest N uptake of 98.3 g plot-1 and 181.1 g plot-1 were observed at rate of 240 kg N ha-1 in addition to zero Gy, compared to control, whereas, under doses 80 Gy the highest values of N uptake were 120.1 g plot-1 and 195 g plot-1 which recorded with straw weight and grains yield, respectively.

Key word: Nitrogen fertilizer, Gamma rays, Wheat.

Full Paper in English: PDF

In vitro Mutagenesis for Increasing Drought Tolerance and Molecular Characterization in Grape (Vitis vinifera L.) cv. “Black Matrouh”

Rehab N. Al-Mousa*(1) Neveen A. Hassan(2) Ramzy G. Stino(3) and Amina H. Gomaa(3)

(1). Department of Biotechnology, General Commission for Scientific Agricultural Research (GCSAR), Damascus, Syria.
(2). National Gene Bank and Genetic Resources, Agricultural Research Center, Giza, Egypt
(3). Pomology Department, Faculty of agriculture, Cairo University, Giza, Egypt

(*Corresponding author: Dr. Rehab N. Al-Mousa. Email: bebo_moussa13@yahoo.com).

Received: 01/08/ 2015                                     Accepted: 29/10/ 2015

Abstract

This study was conducted at the National Gene Bank and Atomic Energy Commission, Egypt started in 2012 to 2014; to produce grape (Vitis vinifera L.) plantlets more tolerant to drought. Shoot tip explants of grape cv. “Black Matrouh” were irradiated with various doses (0-50 Gray) of gamma rays and three vegetative generations (from M1V1 to M1V3) were obtained. To induce drought stress, different concentrations (0, 5 and 10 gl-1) of polyethylene glycol (PEG 6000) were added to the media. The sensitivity of shoot tip explants to radiation increased gradually with increasing gamma rays dose, and LD50 was found at 50 Gray. Elevated shoot number and length were observed with increasing the subculture number. Under drought stress, vegetative and rooting parameters gradually decreased at the same radiation dose with increasing PEG concentration. Explants could tolerate the hard effect of drought when irradiated with 20 Gray of gamma rays. Out of the 20 Inter Simple Sequence Repeat primers used, 6 primers (17898-B, 852, ISSR-35, 834, 889 and HB-9) generated 8 unique bands in plants irradiated with 20 Gray that could be considered as potential markers for initial estimation and selection of drought tolerance in grape.

Keywords: Grape, Gamma rays, LD50, Drought tolerance, PEG, ISSR analysis.

full paper: In vitro Mutagenesis for Increasing Drought Tolerance and Molecular Characterization in Grape (Vitis vinifera L.) cv. “Black Matrouh”.