Test the Pathogenicity of Isolates from Fusarium oxysporum . f. Sp. tuberosi on the Root and Stem of the Potato Plant (Solanum tuberosum L.)

Emad Altinawi(1)*, Fahed Albiski(2) and Jaoudat Faddoul(3)

(1). Department of Biotechnology, General Commission for Scientific Agricultural Research, Syria.

(2). National Commission for Biotechnology (NCBT), Damascus, Syria.

(3). Faculty of Agriculture, Damascus University.

(*Corresponding author: Emad Altinawi E-mail: emademalti66@gmail.com, ).

Received:18/05/2021              Accepted: 14/03/2022


The research was carried out in the laboratories of Plant Protection Research Directorate and the Biotechnology Department of the General Commission for Scientific Agricultural Research during the period from April 2018 to the end of December 2019. The diseased potato plant samples (Solanum tuberosum L.) (cv. Spunta) infected by the Vascular wilt and Dry rot on the tubers were collected from many research centers AL Ghab, Tartous, and Homs, and stations Jmmaza, Jussie, Ghota and Srugaia (AL-Zabadani) of the General Commission of the Agricultural Scientific Research in Syria over two seasons 2018 and 2019. F. oxysporium  had  been defined from  the fungal community, eight isolates of F. oxysporium. f. sp. tuberosi  (FOT) monospores according to Van Dieghem by using the Hanging Drop method. Isolates were identified morphologicaly and microscopically. The pathogenicity tests of the isolates were carried out stem and roots of potato plantlets (cv. Spunta). The data was recorded on four replicate (plantlets) from each treatment in addition to the control after 60 day from infection by standard for the stems and roots between (0-5). The results revealed that isolate FOT23 was the most virulent compared with other isolates, While the isolate FOT28 was the least virulent.

Key Words: Solanum tuberosum, F. oxysporum. f. sp. tuberosi, (cv. Spunta), Pathogenicity tests, Virulence, Fusarium vascular wilt.  

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