Aysar Almemary*(1) Bashar Z. Kassab Bashi(1) and Ayad Chachan Aldaoody(2)
(1). Horticulture and Landscape Department, Faculty of Agriculture and Forestry, Mosul University, Iraq.
(2). Faculty of Education, Mosul, Iraq.
(*Corresponding author: Dr. Aysar Almemary. E-Mail: aysaralsalim@yahoo.com).
Received: 12/12/2017 Accepted: 03/06/2018
Abstract
This investigation was carried out at Tissue Culture Laboratory, Department of Horticulture, University of Mosul, Iraq during (2012-2014) on Catharanthus roseus Variety; Nirvana Pink Blush, which is one of the important medicinal plant, containing a group of alkaloids. Parts of leaves, nodes, and internodes were cultured on MS medium supplemented with NAA for callus initiation, data were taken after 28-day results refer that highest weight of callus; 0.612g, 0.639g ,0.835g were obtained from cultivated parts of leaves, nodes and internodes on MS medium supplemented with 1.0mg/l NAA respectively. Callus was initiated and cultured using parts of leaves, nodes and internodes on MS medium supplemented with BA and 1.0 mg/l of NAA, after 50 days. Vinblastine, vinblastine, vindoline, Ajmalicine were determined by HPLC, the highest absorption value for vinblastine was achieved from callus produced from cultured part of leaf on MS medium supplemented with 0.3 mg/l BA and 1.0 mg/l NAA at retention time of 12.686 with detected area of 955567, and the highest absorption value for vincristine at retention time of 17.940 with detected area of 2658303, from callus produced from cultured internodes on MS medium supplemented with 0.5 mg/l BA and 1.0 mg/l NAA. The highest absorption value for vindoline at retention time of 21.113 with detected area 462847 from callus produced from cultured parts of leaf on MS medium supplemented with 0.1 mg/l BA and 1.0 mg/l NAA. While the highest absorption value for ajmalicine at retention time of 32.510 with detected area 462847, and from callus produced from cultured nodes on MS medium supplemented with 0.1 mg/l BA and 1.0 mg/l NAA
Key words: Catharanthus roseus , Callus, Nods, Alkaloids.
Full paper in Arabic: PDF