Comparison of The Effect of Cogon Grass Extract and Kinetin on Microtubes Production of Two Potato Cultivars Using Tissue Culture

Zainab AL-Hussain*(1) and AbaAljasem AL-Jibboury(2)

(1). Bio Techniques Research Center, Al-Nahrean University, Baghdad, Iraq.
(2). Agricultural and Food Research Directorate, Ministry of Science and Technology,                        Baghdad, Iraq.

(Corresponding author: Dr. Zainab AL-Hussain. E-Mail:

Received: 16/12/2017                    Accepted: 06/07/2018


The present study was conducted at Genetic Engineering Department in Agricultural Research Directorate in Ministry of Science and Technology/Iraq in 2013, to evaluate the effectiveness of aqueous extract of cogon grass at concentrations of (0.0 ,0.25, 0.5, 1, 1.5 and 2%) and Kinetin (0.0, 1.5, 3, 5) mg/L on microtuberization of two potato varieties; Daimond and Desire, in two separate experiments. All cultures were placed in a growth room chamber at temeratures of 18-20 °C with darkness for 90 days, until microtubers harvest. Data of microtuber per plant, diameters and weights were investigated. Results showed a positive effect of cogon grass extract at 2% on the number of microtubers and fresh weight (2.1, 104.41 mg, respectively) and at 1% on the diameter (5.52 mm). Desiree cultivar was the best in fresh weight of microtubers (188.41mg). The results of kinetin showed a significant effect on all of the studied traits. The superior kinetin concentration was 5.0 mg/L which gave the highest number of microtubers 2.22, while the concentrations 3.0 and 1.5 mg/L gave the highest values of diameter and weight ( 6.95 mm and 388.38 mg, respectively). The two cultivars did not differ significantly in number of microtubers, while Diamond cultivar was significantly higher in diameter and weight (6.86 mm and 363.45 mg, respectively).
Key words: Potato, Cogon grass, Aqueous extract, Kinetin, Microtubers.

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