Evaluation of the Susceptibility of some Tomato Hybrids used in Greenhouses of Syrian Coast Against Bacterial Stem Pith Necrosis Disease

Evaluation of the Susceptibility of some Tomato Hybrids used in Greenhouses of Syrian Coast Against Bacterial Stem Pith Necrosis Disease

Abdullatif Al Ghazzawi*(1) Mahmoud Abu Ghoura(2) Nabeel Al Ahmad Bek(1) and Raghda Al Baghdadi(1)

(1). Plant Protection Research Administration, General Commission for Scientific Agricultural Research (GCSAR), Damascus, Syria.
(2). Department of Plant Protection, Faculty of Agriculture, Damascus University, Damascus, Syria.
(*Corresponding author: Eng. Abdullatif Al Ghazawi. E-Mail: Ghazawi11@gmail.com).

Received: 13/12/2015                           Accepted: 28/04/2016

Abstract

Tomato stem pith necrosis disease which is caused by Pseudomonas corrugata Roberts & Scarlettis is one of the most important diseases infecting tomatoes which grown in the greenhouses that spreaded in the Syrian coast region. 40 isolates of P. corrugata were collected from Tartous and Latakia governorates, and their ability of infection, and virulence were evaluated. The most three virulent isolates (G12.1, G14.3 and G35) were chosen to test their infection ability on ten tomato hybrids which most grown in the greenhouses, depending on their susceptibility scale from 0 to 3. The tested isolates were varied in their virulence in Tartous and Latakia, and within the same governorate. Some isolates from Tartous (G35, G14.3 and G12.1) were superior in their ability of infection compared with the rest of isolates collected from both governorates against the plants of the hybrid Code 80 F1, the values of infection were 4.9, 4.7 and 4.4, respectively. Meanwhile, both Tartous isolate 107.1 and Latakia isolate 121.1 were in the last order in their ability of infection. The results showed also that all ten tested tomato hybrids were infected by the bacteria under the artificial inoculation conditions with variable degrees. The hybrid Saida was the highest susceptible with an average of infection 6.61 and the lowest was hybrid Sidra with an average of infection 2.31. The tested hybrids were divided according to the infection scale to high infected, moderate infected, and partially tolerant and tolerant.

Key words: Pith necrosis, Pseudomonas corrugata, Syria, Tomato hybrids.

Full paper in Arabic: Evaluation of the Susceptibility of some Tomato Hybrids used in Greenhouses of Syrian Coast Against Bacterial Stem Pith Necrosis Disease

Preliminary Survey of Some Causal Agents of Tomato Stem Rot and Pith Necrosis in Greenhouses of the Coastal Region in Syria

Abd Al-lateef Al-Ghazzawi*(1) Mahmood Abu Ghoura(2) Nabil Al-Beig(1) and Ragda Al-Baghdadi(1)

(1). Administration Research of Plant Protection, General Commission for Scientific Agricultural Research (GCSAR), Damascus, Syria.
(2). Department of Plant Protection, Faculty of Agriculture, Damascus University, Damascus, Syria.

(*Corresponding author: Abdullatif  Al Ghazawi. E-mail: Ghazawi11@gmail.com. Mobile phone: +963 0991187894).

Abstract

Tomato stem pith necrosis disease has been observed in the greenhouses in Syria since the beginning of nineties in northern west of Aleppo, but recent surveys confirmed the existence of the disease in the coastal region (Tartous and Lattakia governorates), where tomato is cultivated intensively in greenhouses. The results of the survey showed that percentage of greenhouses infected with the disease in Tartous governorate during 2010 and  2011 were 17.7 % and 24.4% respectively, and the disease averages of spread were 0.19% and 0.28% respectively, where infected plants percentages were 0.72% and 0.97% respectively. The percentages of greenhouses infected with the disease in Lattakia governorate during the 2010 and 2011 were 23.20 and 24.86% respectively, and the disease averages of spread were 0.32 and 0.35% respectively, where infected plants percentages were 0.65% and 0.85% respectively. 60 isolates out of 100 isolates resulted from the survey, were characterized morphologically, physiologically and biochemically as Pseudomonas corrugate, where 12 isolates were identified as Pectobacterium carotovorum subsp. Carotovorum, and PCR was used to identify 4 isolates resulted from (pcc) with the aid of specific primers.

Key words: Tomato. Pectobacterium carotovorum subsp. carotovorum, Pith necrosis, Pseudomonas corrugate. Lattakkia, Tartous.

Full paper in Arabic:حصر أولي لمسببات موت لب ساق البندورة في الزراعة المحمية في المنطقة الساحلية من سورية