Tag Archives: PGPR

Effect of Three Species of Rhizobacteria (PGPR) in of Stimulating Systemic Resistance Against Cucumber Mosaic Virus (CMV) of Tomato Plants

Ramez M. Al Shami*(1) Imad D. Ismail(1) and Yaser Hammad(2)

(1). Plant Protection Department, Faculty of Agriculture, Tishreen University, Latakia, Syria.

(2). Soil and Water Department, Faculty of Agriculture, Tishreen University, Latakia, Syria.

(*Corresponding author: Eng. Ramez M. Al Shami. E-Mail: ramezalshami924@gemail.com).

Received: 30/06/2017                              Accepted: 03/10/2017

Abstract

This experiment aimed to study the effect of three species of plant growth promoting rhizobacteria (Frateuria aurantia, Bacillus megaterium and Azotobacter chroococcum) by inoculated seeds or seedlings of tomato plants on suppressed the effect of Cucumber mosaic virus (CMV) in greenhouse in Tartus. Total phenols and photosynthesis pigments (chlorophyll A, B and carotenoids) were observed in fresh leaves of tomato plant after 30 Days of infection. Results showed, that treatment with single bacteria Frateuria aurantiawas was significantly higher in total phenol compared  with Bacillus megateriumor, Azotobacter chroococcum, compared with the control healthy or infected. However, the bacteria Azotobacter chroococcum gave a significant increase in photosynthesis pigments compared with Bacillus megaterium or Frateuria aurantia. On the other hand, the mixed treatment of ABF+CMV (seeds and seedlings) showed highest increase in total phenol (37.75 and 47.09 mg/100g) consequently, photosynthesis pigments (chlorophyll A 0.973 and 1.065 mg/g), (chlorophyll B 0.872 and 0.903mg/g) and (carotenoids 0.972 and 0.995 mg/g) respectively. The increase in total phenols and photosynthesis pigments leads to the ability ofrhizobacterto stimulate systemic resistance and reduce the effect of the virus on tomato plants.

Keywords: CMV, PGPR, Tomato, Total phenols, Photosynthesis pigments.

Full paper in Arabic: PDF

Effect of Seed Treatments of Tomato Variety Merel with Four PGPR Bacterial Strains on Promoting Peroxidase Enzyme Activity and Growth Improvement

Hanan Kawas *(1) Omar Hamudi(2) Ahmad Ahmad(3) Imad  Ismail(4)

(1). Department of Plant Protection, Faculty of Agriculture, Tishreen University, Lattakia, Syria.

(2). Agricultural Research Center of Latakia, General Commission for Scientific Agriculture Research (GCSAR), Damascus, Syria.

(3). Agricultural Research Center of Tartous, (GCSAR), Damascus, Syria.

(4). Department of Plant Protection, Faculty of Agriculture, Tishreen University, Lattakia, Syria.

(*Corresponding author: Eng. Hanan Kawas. E-Mail: hanankawas1@gmail.com).

Received: 18/12/2016                           Accepted: 14/02/2017

Abstract

This research was conducted to study the effect of four bacterial strains (PGPR): Pseudomonas chlororaphis MA342, Serratia plymuthica HRO-C48, Bacillussubtillis B2g and B. subtillis FZB27 to improve the growth of tomato plants in the greenhouse. Bacterial strains were applied to the seeds in a concentration of (1010 cfu/ml), of each bacterial strain. The results showed the treatment with bacteria improved peroxidase enzyme activity at a rate of (2.15-6.35) % after 78 days of treatment compared with non-treated control (0.002) n mol, and a rate of (60.71- 232.14) % after 94 days compared with non-treated control (0.056) n mol. The treatment with bacteria increased the height of plant at a rate of (1-21.37)% compared with non-treated control (57.83) cm, and increased fresh weight of foliage at a rate of (9.25-122.56)% compared with non-treated control (317)g, while it increased dry weight of foliage at a rate of (3.89- 61.98)% compared with non-treated control (111.33)g, and increased fresh root weight at a rate of (22.39-310.48)% compared with non-treated control (22.33)g, and increased root dry weight at a rate of (9.09- 33.27)% compared with non-treated control (5.5)g, and increased number of fruits at a rate of (7.73-54.73)% compared with non-treated control (8.66 fruit), and increased weight of fruits at a rate of (5.69-60.33)% compared with non-treated control (105.33)g . The strain B27 had the highest values of the studied traits.

Key words: PGPR, Pseudomonas chlororaphis MA342,  Serratia plymuthica HRO-C48,  Bacillus subtillis B2g,  B. subtillis FZB27,  Tomato.

Full paper in Arabic: PDF