Tag Archives: Osmotic potential

Effect of Osmotic Potential Induced by Polyethylene Glycol (PEG6000) on the Germination of Five Chickpea (Cicer arietinum L.) Varieties

Loubana Al-Saleh (1) Bashar Hyass (2) and Fadi Abbas*(3)

(1). Department of Field Crops, Faculty of Agriculture, Al Baath University, Homs, Syria.

(2). Agriculture Research Center of Homs, General Commission for Scientific Agricultural Research (GCSAR), Damascus, Syria.

(*Corresponding author: Dr. Fadi Abbas. E-Mail: fadiab77@gmail.com).

Received: 01/12/2018                                Accepted: 10/01/2019

Abstract

This experiment was conducted in the labs of Chemistry and Petroleum Engineering Faculty at AL-Baath University, Homs, during 2017. PEG6000 was applied to the germination media with low osmotic potentials (OP) i.e. 0, -3 and -6 bar, for screening 5 chickpea varieties under controlled conditions. Complete randomized design (CRD) with three replicates was used. The results exhibited a genetic difference between the varieties concerning the response to the application of PEG6000 in the media. The germination percent was decreased with the decline of osmotic potential 0, -3 and -6 bar. The percentages of germination were 100, 93.67 and 70.73 % respectively, as compared with the control. While the speed of germination was 6.70, 4.98 and 2.22 respectively. The length of epicotyl and radicle were estimated in all of tested osmotic potentials, and the results showed that with decreasing the osmotic potential the length decreased, and the decrement in epicotyl was more than radicle. The variety Ghab5 achieved the lowest scores in growth declaim compare to the other varieties. The differences between genotypes in the response to drought stress using PEG6000 during germination, and the z-distribution analysis whish classified the varieties to tolerant and sensitive. Ghab5 and Ghab4 were considered as tolerant genotypes for drought stress, and could be sown successfully under the most drought conditions, compare to other varieties.

Key words: Osmotic potential, Polyethylene Glycol PEG6000, Germination stage, Chickpea.

Full paper in Arabic: PDF

Evaluation of Some Morpho-Physiological Criteria in Wheat Genotypes to Osmotic Potential  by Polyethylene Glycol (PEG6000)

Ghassan Al Lahham*(1)    Zaynab Tadbeer(1)    Reem Al Mansour(1)  Razan Al Najjar(1) Riad Balish(1)  Mohammed  Ali (1) Saoud Shehab(1) and Thamer Al Henish(1)

(1). Crops Research Administration, General Commission for Scientific Agricultural Research (GCSAR), Damascus, Syria.

(*Corresponding author: Dr. Ghassan Al Lahham. E-Mail: gh_lahham@hotmail.com).

Received: 26/08/ 2015                                     Accepted: 09/09/ 2015

Abstract

The experiment was carried out at the General Commission for Scientific Agricultural Research (GCSAR), Damascus/Syria, in 2012, to assess the performance of 15 genotypes of wheat at germination and seedling stages, under artificially imposed of three osmotic potential levels (-0.6, -0.8, -1 MPa), using specific concentrations of poly ethylene glycol PEG6000, in addition to the control, according to splitplot design, according to plant growth stage, with three replicates. The results showed significant differences (P≤0.01), among the genotypes. The genotypes (Hourani , Bohoth 7, Douma 1, Douma 41282, Douma 45367, Douma 4, Golan 2, h-8150) achieved the lowest reduction in relative drought tolerance index (RDTI) about (4, 2, 1, 8, 12, 9, 5, 3%) respectively. The media of osmotic potential caused a significantdecline in growing seedling, but the  genotypes  (Douma 1, HouraniBohoth 7, Douma 41282,Bohoth 8, Golan 2) had the highest seedling superioritywitha remarkableincreaseindry matteraccumulation(DMSI%)at the high level (-1MPa)  about (36، 30، 42 ، 43، 34، 34، 36، 43، 48%) respectively, compared with control. and classified as more tolerance for osmotic potential. A significant correlation between physiological  indicators was determinedwhich appear the importance ofrelative drought tolerance index, duringseed germination,whichreflected onroot lengthincrementlater (r=0.608 **) and havingstrong (r=0.743 **) under stress conditions, during the early growth stages of wheat.

Keywords: Osmotic potential, PEG6000, Relative drought tolerance index, Wheat.

Full paper in Arabic:

تقييم بعض المعايير المورفو-فيزيولوجية في تحمل طرز من القمح للإجهاد الحلولي باستخدام بولي إيثيلين غليكول  (PEG6000)