Evaluation of Some Morpho-Physiological Criteria in Wheat Genotypes to Osmotic Potential  by Polyethylene Glycol (PEG6000)

Ghassan Al Lahham*(1)    Zaynab Tadbeer(1)    Reem Al Mansour(1)  Razan Al Najjar(1) Riad Balish(1)  Mohammed  Ali (1) Saoud Shehab(1) and Thamer Al Henish(1)

(1). Crops Research Administration, General Commission for Scientific Agricultural Research (GCSAR), Damascus, Syria.

(*Corresponding author: Dr. Ghassan Al Lahham. E-Mail: gh_lahham@hotmail.com).

Received: 26/08/ 2015                                     Accepted: 09/09/ 2015

Abstract

The experiment was carried out at the General Commission for Scientific Agricultural Research (GCSAR), Damascus/Syria, in 2012, to assess the performance of 15 genotypes of wheat at germination and seedling stages, under artificially imposed of three osmotic potential levels (-0.6, -0.8, -1 MPa), using specific concentrations of poly ethylene glycol PEG6000, in addition to the control, according to splitplot design, according to plant growth stage, with three replicates. The results showed significant differences (P≤0.01), among the genotypes. The genotypes (Hourani , Bohoth 7, Douma 1, Douma 41282, Douma 45367, Douma 4, Golan 2, h-8150) achieved the lowest reduction in relative drought tolerance index (RDTI) about (4, 2, 1, 8, 12, 9, 5, 3%) respectively. The media of osmotic potential caused a significantdecline in growing seedling, but the  genotypes  (Douma 1, HouraniBohoth 7, Douma 41282,Bohoth 8, Golan 2) had the highest seedling superioritywitha remarkableincreaseindry matteraccumulation(DMSI%)at the high level (-1MPa)  about (36، 30، 42 ، 43، 34، 34، 36، 43، 48%) respectively, compared with control. and classified as more tolerance for osmotic potential. A significant correlation between physiological  indicators was determinedwhich appear the importance ofrelative drought tolerance index, duringseed germination,whichreflected onroot lengthincrementlater (r=0.608 **) and havingstrong (r=0.743 **) under stress conditions, during the early growth stages of wheat.

Keywords: Osmotic potential, PEG6000, Relative drought tolerance index, Wheat.

Full paper in Arabic:

تقييم بعض المعايير المورفو-فيزيولوجية في تحمل طرز من القمح للإجهاد الحلولي باستخدام بولي إيثيلين غليكول  (PEG6000)