Isolate and Identify of Storage Fungi in Two Varieties of ‎Peanut (Arachis hypogaea L.) and Detection their Ability for ‎the Toxins Secretion ‎

Magida Younis El-Kadi(1) and Zahra Ibrahim El-Gali*(1)

(1). Department of Plant Protection, Faculty of Agriculture, Omer Al-Mukhtar University, Libya.

(*Corresponding author: Dr. Zahra Ibrahim El-Gali. E-Mail:

Received: 31/03/2020                               Accepted: 21/05/2020


In the present study, seeds of tow groundnut varieties viz. Landraces (local vs.) and Virginia were collected from different market places of El-Beida city, Libya, and seed mycoflora was isolated by standard blotter paper method and agar plate method, then identified and addition to be checked for toxin production on PSA, CMA and YSA solid media. The identified fungal isolates included Aspergillus flavus, A. niger, A. terreus, Cldosporium cladospoirides, Fusarium sp. and Penicillium italicum. Data revealed that both varieties were affected by the fungal species however, 30 % of A. niger on agar plate method and 25 % on standard blotter paper, while A. terreus had the least percent incidence of up to 2% on agar plate method. Large seeds were more prone to fungal contamination than small seeds and higher numbers of fungi were isolated on agar plate method used as compared to standard blotter paper method. Results of the ability of fungal isolates for secretion toxins after exposure to liquid ammonia were recorded that color changed in pigment with different intensities, and PSA medium was suitable for toxin secretion by A. flavus, A. niger and P. italicum.

Keywords: Storage fungi, Incubation tests, Toxins secretion, Peanut, Libya.

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