Abeer jubily*(1) and Fahed Albiski(2)
(1). General Commission for scientific and agriculture research, Lattakia, Syria.
(2). National commission for biotechnology, Damascus, Syria.
(*corresponding author: Dr. Abeer Jubily. E-Mail; abeerjubily.e@gmail.com).
Received: 18/06/2024 Accepted: 2/09/2024
Abstract:
This experiment was carried out at the Agricultural Science Research Center in Lattakia- General Combition for Scientific Agricultural Research, Syria during seasons 2017-2022.The mutagens gamma rays and ethyl methanesulfonate (EMS) were used to induce genetic variation in chickpea cultivar Gab.5. DNA was amplified using ten primers (RAPD) of 17 promising mutant families of Gab (5) selected based on morphological and productive characteristics compared to the control. The results showed genetic differences between 7 mutant families derived from Ghab5 compared to the control. The primers resulted in a total of 81 bands, with an average polymorphism of 22.82%. Cluster analysis based on the RAPD results divided the cultivar Gab.5 and the selected families into seven different groups. The availability of the desired mutations gives a good opportunity to develop improved chickpea cultivars in addition to generating genetic variation, which enriches the genetic resources of chickpea.
Key words: Cicer arietinum L., genetic variation, RAPD technique, dendrogram.
Full paper in Arabic: pdf