Yanal Al-Kuddsi(1)*, Shahinaz Abbas(1), Alaa Al-Shaal(1), Rihab Al-Mousa(1), Tony Saloom(1), Khouzama Kountar(1) and Nabila Ali Basha(1)
(1). Department of Biotechnology, General Commission for Scientific Agricultural Research, Damascus, Syria.
(* Corresponding author: Yanal Al-Kuddsi. E-mail: y.alkuddsi@gmail.com)
Received: 21/06/2021 Accepted:13/08/2022
Abstract:
A Collection of 11 Syrian barley varieties (Fourat1, Fourat2, Fourat3, Fourat4, Fourat5, Fourat6, Fourat7, Fourat9, Arabi Abiad, Arabi Aswad, Arabi Abiad Mouhassan) were studied during the period of 2019-2021, and the genetic diversity among them was determined using ISSR technique. 12 primers were used, all of which gave amplification products in PCR polymerase chain reaction. These primers proved their discriminative power and gave a total of (120) bands with an average of (10) bands/ primer, of which 90 bands were polymorphic, and the PIC values ranged between (0.08 – 0.32) with an overall average of (0.25). Results showed that there is a significant genetic diversity among the studied varieties, as the UPGMA (unweighted pair group method with arithmetical averages) clustering based on ISSR data grouped the 11 varieties into two main clusters, and the highest degree of genetic diversity was between Fourat1 and Fourat6.
Key words: ISSR (Inter Simple Sequences Repeat), Genetic diversity, Syrian barely varieties.
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