ISSR Markers
Rehab Al-Mousa* (1), Alaa Alshaal (1), Shahinaz Abbas (1), Nabila Ali Bacha (1), Yanal Al-Kudssi (1), Khozama Kountar (1) and Tony Saloom(1)
(1). Department of Biotechnology, General Commission for Scientific Agricultural Research, Damascus, Syria.
(*Corresponding author: Dr.Rehab Al-Mousa, e-mail: bebo_moussa@yahoo.com, )
Received: 27/02/2022 Accepted: 26/07/2022
Abstract:
The present research was conducted to detect the genetic relationship among twelve lentil lines introduced from ICARDA and two local varieties. Eighteen Inter Simple Sequence Repeats (ISSR) primers successfully amplified 167 bands in all genotypes with the average of 9.27 bands/primer. The mean value of polymorphism percentage was 78.7%. Primers NLSSR3 and 830 presented 100% polymorphism percentage. The highest number of amplification bands (124) was noticed in genotype (Ln12), while the lowest number of amplification bands (87) was noticed in genotype (Idleb5). Thirty one unique bands were obtained, out of them 9 were considered as present bands (positive) and 22 were considered as absent bands (negative). The highest number of unique bands (5) were registered in genotype (Idleb3), while genotypes (Ln9 and Ln12) did not present any unique band. The genetic distance ranged from 0.21 to 0.48. ISSR cluster analysis grouped the genotypes into two main clusters; the first one included local varieties (Idleb3 and Idleb5), while the second cluster included the remaining lines. Some genotypes showed wide divergence (Idleb5, Ln1 and Ln6), whereas genotypes (Ln11 and Ln 12) were close related.
Keywords: Lentil, ICARDA lines, local varieties, genetic relationship, ISSR analysis.
Full paper in English: pdf