Effect of Aqueous Extract of Stem Euphorbia helioscopia on Mitotic Division of Chickpea (Cicer arietinum L.)

Fatima Alzhraa Enjrini(1)*, Imad Aldeen Alkhalaf (1), Walid AlSaid (1)

  • of Botany, Faculty of Science, University of Aleppo, Aleppo, Syria.

(*corresponding author: Fatima Enjrini E-Mail: fatimaenj92@gmail.com)

Received: 19/10/2020        Accepted: 25/08/2020

Abstract

Biological relations play an important and essential role in the composition of the components of ecosystems especially agricultural, Allelopathy is one of the most important of these relations, the research was conducted in the Laboratory of plant Genetics Research at the Department of Botany / Faculty of Science / University of Aleppo 2019 year. The aim of this research is to study the effect of the aqueous extract of the stem of Euphorbia helioscopia with concentrations (5, 10, 20, 40%) on the mitotic division of chickpea. The results showed a decrease in the mitotic ndex of the low concentrations of aqueous extract (5,10)%, and increased with increasing concentration (20,40)%, this was reversed and led to an increase in the proportion of chromosomes aberration, reaching to (30.8)% at concentration 40% compared with the control (3.11)%, and the following chromosomes aberration appeared: Stickiness, Breakage in Metaphase, Chromosomal Bridges multipolar, the late separation, Polarization, Binucleate, The late separation, Polarization, Binucleate, This indicates that the Euphorbia helioscopia plant that was used in this study and that it contains chemicals that are phytotoxic toxic substances. Hence, the Euphorbia helioscopia plant with its water extract of the stem can be considered a mutagen.

Keywords: Allelopathy, Euphorbia helioscopia, Mitotic, Chickpea, Chromosomal aberration

Full paper in Arabic: PDF