Bachar Aldakil^*(1)

(1). Department of Plant Protection, Faculty of Agriculture, Aleppo University, Aleppo, Syria.

(*Corresponding author: Dr. Bachar Aldakil. E-Mail: bashardakhel6@gmail.com).

Received: 11/12/2019                               Accepted: 21/01/2020

Abstract

Tomato plants are exposed to many fungal, bacterial and viral diseases, affecting their quality and quantity. Tomato wilt disease caused by the fungus Fusarium oxysporum f.sp. lycopersici is an important disease. The aim of the search was to study the effect of bacterial and fungal isolates, in  single and combination treatments, that belong to the genera of Basillus sp. (Isolation 1) and Pseudomonas sp. (Isolation 3) and Trichoderma sp. (Isolation 2), which was proven in vitro, by treating tomato seeds, a susceptible local variety before planting with bacterial suspensions at a concentration of 1 x 108 CFU/ml, or treatment of sterilized potted soil with a spore suspension of Trichoderma sp. (2) at a concentration of 1 x 107 spores/ml after planting the seedlings at the age of 3 real leaves directly. The soil was infested by adding a content of a 9 cm diameter Petri dish of a 10-days colony of pathogenic fungus (mycelium and spores) per treatment /4 pots, 6 days after the addition of Trichoderma sp. Isolate, and the results were taken at the beginning of flowering. The results showed that there were highly significant differences between the treatments when comparing the mean wet weight of the vegetative parts, as the combined treatment Pseudomonas sp. (3) Trichoderma sp. (2) significant differences with all other treatments, as the mean wet weight of the vegetative parts compared to the treatment of the infectious control which was 29 and 10.22 g, respectively. Likewise, the highest value of the average wet weight of the root total was recorded at the same treatment where the average wet weight of the root total was 66.72 and 22.82 g, respectively. The results of this study indicated the possibility of some isolates of bacterial and fungal organisms with opposite ability to reduce the damages of the fungus that causes tomato wilt disease by improving plant growth.

Keywords: F.oxysporum f.sp lycopersici, Tomato, Plant growth enhancement, Biocontrol.

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