Daniel Al-Awad⁽¹⁾ Maysaa Yazigi⁽¹⁾ and Reem Ebraheem^*(1)

(1). Department of Plant Biology, Faculty of Sciences, Tishreen university, Latakia, Syria.

(*Corresponding author: Reem Ebraheem, E-mail: ribraheem66@yahoo.com).

Received: 02/04/2018                                Accepted: 19/06/2018

Abstract

This study investigated the effect of some plant growth hormones on the shoots formation and callus induction of Nasturtium officinale R.Br, in vitro. All experiments were carried out at the laboratory of Department of Plant Biology, Faculty of Science, Tishreen University during 2016 and 2017. Seedlings were grown on MS medium, then 0.5-1 cm of stem nodes were planted on the MS nutrient medium with 0.1, 0.5, 1, 2 and 4 mg/l of the cytokinin Benzyl Amino Purine (BAP). Stem nodes, leaves and petioles of leaves were planted on MS medium with various concentrations (1, 2) mg/l of the auxin 2,4-dichlorophenoxy acetic acid (2,4-D) in combination with various concentrations (0.1, 0.5, 1) mg/l of BAP. The cultures were maintained in growth incubator at temperature 25±2 °C with a photoperiod of 16 hours light (2500-3000 lux) and 8 hours dark. The results showed that MS medium with 0.1 mg/l or 0.5 mg/l BAP was the best to propagate buds, where the average number of shoots ranged between 14-15, and some shoots formed roots. The MS medium supplement with 1mg/l of 2,4-D in combination with 0.5 mg/l BAP was  the best medium for callus induction from all parts of the plant, and the stem nodes were the best plant parts used to form callus on MS medium in most of hormone concentrations; stem nodes recorded best rates of callus formation 83.75% then the petioles of leaves was 75% and the leaves was 66.75%, on the best medium.

Keywords: Nasturtium officinal R.Br, Tissue culture, Auxin, Cytokinin, Callus.

Full Paper in Arabic: PDF