Mai Al Maghout*(1) Wade Shadid(1) and Samer Hamud(1)
(1). Department of Pathology. Faculty of Veterinary Medicine, Hama University, Hama, Syria.
(*Corresponding author: Vet. Mai Al Maghout. E-Mail: aya.kanawaty87@gmail.com).
Received: 10/12/2017 Accepted: 21/02/2018
Abstract
The research was carried out on 70 birds of two-week-old broiler, using a well-known strain (Habre flex), which is disease-free strain, at a poultry farm near Salamieh province in 2105. The research was divided into two main stages: the first stage was divided into five groups, using 50 birds, which were treated with five doses of levamisole (0, 0.5, 1, 1.5 and 2) g/kg respectively, to determine (LD50). The second stage used 20 birds, which were divided to tow groups (10 birds for each). The first group was considered as control (water only), while the second group was given the LD50 of levamisole that was calculated in the first stage. Different tissues samples of the birds were taken and preserved in neutral formalin, and then a tissue slices were prepared and dyed with hematoxylin and Eosin. The LD50 levamisole value was determined according to local conditions of breeding in Salamieh by (LD50=1g/kg) of the bird’s weight. Before the death of the birds because of LD50 of levamisole, it was noticed some symptoms such as convulsions and dyspnea which they were not seen in the control. These symptoms were as a result of levamisole effect on cholinergic recipients. The histological study also showed that during severe toxicity of levamisole, it was noticed a microscopic changes in liver and kidney. The changes were in the form of necrosis foci and infiltration of inflammatory cells in cellular destruction sites. But the other examined tissues showed no changes. Because of that, it is necessary to use levamisole with extreme care, whether in the treatment of parasites or for immunity motivation, taking into consideration the toxic dose as showed by the study.
Keyword: Anatomical Changes, Levamisole, Broiler.
Full Paper in Arabic: PDF