Ali Sbieh*¹, Mohamed Matar², Qusay Alrhayeh¹ and  Wafaa Choumane³

(1) Agricultural scientific research centre in Lattakia. Syria. 

(2) Department of plant protection, Faculty of Agricultural engineering, Tishreen University, Lattakia. Syria.

(3) Biotechnology Centre, Tishreen University, Lattakia. Syria.

(*Corresponding author: Ali Sbieh, E-Mail: ali_sbeeh@hotmail.com)

Received:   19/3/2024         Accepted:  23/6/2024

Abstract

This study aimed to assess the efficiency of local isolates of the fungus Trichoderma sp. and the bacteria Bacillus subtilis in the biological control of Fusarium wilt disease of tomato, caused by Fusarium oxysporum f. sp. lycopersici ‎‏ (‏FOL‏), in vitro and in the greenhouse. This research conducted in the Agriculture Research Centre in Lattakia during the years 2021 and 2022. The results showed the ability of the used isolates to inhibit the growth of Fusarium in in vitro, by 50% (by Bacillus subtilis) and 33% (by Trichoderma sp) on PDA medium. The results of planting in pots in greenhouse showed the ability of the fungus Trichoderma sp. and the bacteria Bacillus subtilis isolates to reduce the wilt severity percentage and to promote the growth of tomato plants compared to healthy and infected controls. The greenhouse trial confirmed the efficiency of tested isolates in decreasing the disease symptoms and increasing plant growth and production by 209.5%, by Bacillus subtilis isolate B.FZB27, compared to infected control.

Key words: Tomato, Biological control, Bacillus, Trichoderma sp, Fusarium oxysporum f. sp. lycopersici,

  Full paper in Arabic: PDF