Mohammad Naddaf (1),Nisreen Naksho (2) and Amany younes*(1(
(1). Department of Food Science, Faculty of Agriculture Engineering, Tishreen University, Lattakia, Syria.
(2). National Commission for Biotechnology, Faculty of Agriculture Engineering, Damascus University, Syria.
(*Corresponding author: Amany Younes, amanyyounes996@gmail.com ).
Received: 19/02/2024 Accepted: 26/06/2024
Abstract:
This research was carried out in the laboratories of the National Commission for Biotechnology at the University of Damascus during the period 2022-2023. The purpose of this research was to find the optimal fermentation conditions to produce the highest possible yield of biomass from Saccharomyces cerevisiae using carrot juice as the sole carbon source. The Response Surface Methodology (RSM) method was used to determine the best conditions to get the highest yield, where the biomass of the yeast has reached (22.54 g/L) after 24 hours of fermentation, in the following optimal conditions (temperature 30 0C), pH (5), at a rotation speed of 200 rpm during the fermentation process. According to the Syrian standard, the fermentation power of the resulting biomass was excellent, as the volume of CO2 produced reached 2900 (cm3).
Key Words: baker`s yeast (Saccharomyces cerevisiae), Biomass, Carrot juice, Temperature, pH.
Full paper in Arabic: PDF