Protection of Nephrotoxicity Caused by Anti-Cancer Agents in ‚ÄéHamster Using Some Nutrition Compounds

Fadi Al-Mohammad*(1) and  Amad Hamdi Mokresh(2)

(1). Department of Pathology, Faculty of Veterinary Medicine, Hamah University, Syria.

(*Corresponding author: Fadi Al-Mohammad. E-mail: fadi71985@gmail.com).

Received: 06/09/2019                               Accepted: 17/10/2019

Abstract

The experiment was conducted on (108) of adult hamsters, at the laboratory of pathology in the Faculty of Veterinary Medicine at the University of Hama, with the aim of studying the effect of honey, ginseng and vitamin E on nephrotoxicity after peritoneal injection of anti-cancer agents (doxorubicin and cisplatin) in hamsters, and to determine the extent of preventive effect for these materials on nephrotoxicity. The ages of hamster ranged from 3.5 to 4 months and their weights between 60-70 grams. The experimental animals were divided into nine groups, (12) hamsters in each group, according to the following: G1 (control), G2 (injection of anti-cancer without oral administration of any substance), G3 (anti-cancer injection + oral administration of honey), G4 (anti-cancer injection + oral administration of vitamin E), G5 (anti-cancer injection + oral administration of ginseng), G6 (anti-cancer injection + oral administration of honey and vitamin E), G7 (anti-cancer injection + oral administration of honey and ginseng), G8 (anti-cancer injection + oral administration of ginseng and vitamin E), G9 (anti-cancer injection + oral administration of honey, ginseng and vitamin E). The experiment continued for 6 weeks, after peritoneal injection of anticancer agents. Clinical symptoms were studied, and blood samples were taken from the lateral venous plexus of the eye at the end of the 6th week to measure serum creatinine by Jaffe’s reaction method, then the anatomy of hamsters were executed to study histopathological changes of the kidney. The peritoneal injection with anti-cancer agents led to many clinical symptoms such as diarrhea, neurological disorders, loss of appetite and hair loss. The blood changes were in the form of high serum creatinine level which indicated to the damage of kidney and low glomerular filtration rate, but the histopathological changes were in the form of necrotic epithelial cells lining the renal tubules, necrosis of the renal glomerules and loss of their structure and building. It was also noted that oral administration of protective materials (honey, ginseng and vitamin E), led to the reduction of clinical symptoms, reduction of creatine levels, and preservation of the kidney histological structure in experimental animals. Therefore, The study recommends the use of these preventive materials during treatment with anti-cancer agents.

Key words: Anti-cancer agents, Nephrotoxicity, Hamster.

Full paper in Arabic: PDF