Effect of Several Factors on Micrografting of Navel Orange Using Different Rootstocks

Kinda Ibrahim*(1) Ali Al-Khateeb(1) Fahd Al-biski(2) and Khalil Al-Maarri(3)

(1). Latakia Research Center, General Commission for Scientific Agricultural Research (GCSAR), Damascus, Syria.

(2). General Commission of Biotechnology, Damascus, Syria.

(3). Horticulture Department, Faculty of Agriculture, Damascus University, Damascus, Syria.

(*Corresponding author: Eng. Kinda Ibrahim. E-Mail: kindaib@yahoo.com).

Received: 31/05/2016                           Accepted: 19/06/2016

Abstract

The research was conducted at Faculty of Agriculture, Damascus University/Syria, with the help of Scientific Agricultural Research Center in Latakia during 2013 and 2014seasons. Three rootstocks were used i.e. Sour orange, citrumelo and troyer citrange, to study some factors affecting the success of micrografting technique (concentrations and periods of sterilization, rootstock, shoot tip size, type of nutritive medium and the grafting method). The nutritive medium was prepared, and the shoot tips was isolated from Navel orange source, then the micrografting was done for the three rootstocks. The results showed that contamination and germination of seeds were affected by the concentration and period of sterilization. Also, this effect varied among the three rootstocks that were used. Success of grafting depended on the rootstocks. Citrumelo rootstock gave the highest grafting rate (54.17 %). Regarding shoot tip size, the results revealed that this character played a crucial role for the success of micrografting. The highest grafting rate was achieved (62.50%) by using shoot tips of about 1 mm sized. Also, the results showed that grafting method and nutritive medium had vital role on the success of micro-grafting. Micro-grafting rates were better by using liquid medium (45.83%) and with the application of inverted T-incision method (75%).

Key words: Navel orange, In vitro micrografting, Rootstocks.

Full paper in Arabic: PDF

Effect of Seven Citrus Rootstocks on Growth and Production of Washington Navel 141Orange

Alaa S. Ibrahim(1), Eshraq S. Ali(1), Raed N. Sbeeh(1), Rafeek A. Abboud(1)&Fadel S. Alkaiem(2)

(1). Ciano Research Station, Scientific Agricultural Researches Center of Lattakiah, Lattakia, Syria.
(2). Scientific Agricultural Researches Center of Lattakiah, Lattakia, Syria.

(Corresponding author: Alaa S. Ibrahim: Ciano Research Station, Scientific Agricultural Researches Center of Lattakiah, Lattakia, Syria. E-mail: alaasoeb@gmail.com).

                          Received: 13 / 07 / 2014               Accepted: 07 / 12 / 2014

Abstract

The study was carried out during 2011-2012 at Ciano citrus experimental station. Growth and yield of 22 years old orange trees (Washington Navel 141) budded on seven citrus rootstocks (Sour orange, Troyer citrange, Carrizo citrange, Citrumelo 4475, Citrumelo 1452, Macrophylla and Cleopatra mandarin) have been studied. The results showed that Sour orange gave the highest yield (79 kg/tree). Citrumelo 4475 significantly showed the highest trunk section area of the rootstock (779.88 cm2); the highest percentage of fruit juice was achieved by Citrumelo 4475 and Troyer citrange (43.43%, 42.37%) respectively, which were significantly superior to Cleopatra (37.39%). The highest percentage of total soluble solids was achieved by Cleopatra (13.1 %), while the highest TA% achieved by Citrumelo 4475 (0.88%) without significant differences compared to other treatments.

Keywords: Navel orange, Citrus rootstocks, Production, Sour orange, Citrange, Citrumelo.

Full paper in Arabic: تأثير سبعة أصول من الحمضيات في نمو وإنتاج صنف البرتقال أبو صرة (Washington Navel 141)