Kinda Ibrahim*(1) Ali Al-Khateeb(1) Fahd Al-biski(2) and Khalil Al-Maarri(3)
(1). Latakia Research Center, General Commission for Scientific Agricultural Research (GCSAR), Damascus, Syria.
(2). General Commission of Biotechnology, Damascus, Syria.
(3). Horticulture Department, Faculty of Agriculture, Damascus University, Damascus, Syria.
(*Corresponding author: Eng. Kinda Ibrahim. E-Mail: kindaib@yahoo.com).
Received: 31/05/2016 Accepted: 19/06/2016
Abstract
The research was conducted at Faculty of Agriculture, Damascus University/Syria, with the help of Scientific Agricultural Research Center in Latakia during 2013 and 2014seasons. Three rootstocks were used i.e. Sour orange, citrumelo and troyer citrange, to study some factors affecting the success of micrografting technique (concentrations and periods of sterilization, rootstock, shoot tip size, type of nutritive medium and the grafting method). The nutritive medium was prepared, and the shoot tips was isolated from Navel orange source, then the micrografting was done for the three rootstocks. The results showed that contamination and germination of seeds were affected by the concentration and period of sterilization. Also, this effect varied among the three rootstocks that were used. Success of grafting depended on the rootstocks. Citrumelo rootstock gave the highest grafting rate (54.17 %). Regarding shoot tip size, the results revealed that this character played a crucial role for the success of micrografting. The highest grafting rate was achieved (62.50%) by using shoot tips of about 1 mm sized. Also, the results showed that grafting method and nutritive medium had vital role on the success of micro-grafting. Micro-grafting rates were better by using liquid medium (45.83%) and with the application of inverted T-incision method (75%).
Key words: Navel orange, In vitro micrografting, Rootstocks.
Full paper in Arabic: PDF