Bassam Al-Oklah * (1) Jalal Fandi (2) Rudwan Badr Al-Deen (1)
Nour Haj Masoud (1) Lamyaa Husamo (1)
(1). National Commission for Biotechnology, Damascus, Syria.
(2). Faculty of Pharmacy, Damascus University, Damascus, Syria.
(*Corresponding author: Dr. Bassam Al-Oklah, E-Mail: bassam780alk@gmail.com, phone: 0938994115).
Received: 1/05/2023 Accepted: 9/03/2024
Abstract:
This paper aimed to prepare efficient extracts from Thuja orientalis plant leaves by using several solvents (ethyl acetate, methanol, acetone), and study their total phenolic and flavonoid content, their antioxidant activity, and antibacterial activity against gram positive bacteria (Staphylococcus saprophyticus, Staphylococcus aureus, Staphylococcus hominis) and gram negative bacteria (Enterobacter cloacae, Klebsiella oxytoca, E.coli). Their phenol and flavonoid contents were evaluated by Folin-Ciocalteu regent method and aluminum chloride method respectively. Their antioxidant activities were determined by estimating scavenging capacity towards DPPH free radical. And their antibacterial activities were estimated by disc diffusion method. The results showed that the leaf extract prepared from acetone reached the highest content of phenols (26000 mg/100g) and flavonoids (295mg/100g), and the highest activity of scavenging DPPH free radical (75%). The extracts of Thuja orientalis plant leaves showed high antibacterial activities against gram negative bacteria comparing with gram positive ones, and the highest antibacterial activity was against Klebsiella oxytoca.
Keywords: Thuja orientalis, antibacterial activity, antioxidant activity, phenols, flavonoids.
Full paper in Arabic: PDF