Zahraa Baidaq (1)* and Al-Mouthana Hasan(1)
(1). General Commission for Scientific Agricultural Research (GCSAR), Latakia, Syria.
(*Corresponding Author: Dr. Zahraa Baidaq zahraaok2@hotmail.com).
Received: 13/08/2023 Accepted:28/01/2024
Abstract:
Some experiments were conducted on the use of Sliced onion (Allium cepa L.) that were distributed in different places in ambient conditions, as they were hanged at a height of 1.5 m, in order to identify the bacterial species adsorbed on the surface of the onion slices, The onion slices were placed with a diameter of 2.5 cm In 3 different places in terms of the nature of the use of the place, with an average of 3 replications in each of (office room _ living room _ public bathroom), and after three days of hanging, samples were drawn, numbered, and transferred to the laboratory for bacterial examination in the Supply Directorate in Lattakia to study the following criteria: ( The total count of bacteria – special tests for some types of pathogenic bacteria). The most important media that were used in the research were: Nutrient Agar (NA), Red Bile Glucose Agar Violet (VRB), Baird Parker Agar, and Salmonella Tetrathionate Broth. The samples were incubated at specific temperatures suitable for the types of target bacteria to be identified, ranged between 37-44 oC and was incubated for 24 and 48 hours. The results showed that the samples were hanged in office and living rooms, and public bathrooms, and within only three days; It had a high bacterial count compared to the control (samples were taken for direct digestion), as it reached the highest 3.2× 103 bacteria per 1g of the bathroom samples, While the lowest was 3.0× 103 germs per 1g in the living room sample on Nutrient Agar NA, while the control contained 5.0 × 102bacteria per 1 g, while the office and living room samples contained types of coliform group bacteria, including Escherichia coli, while all samples were free of Staphylococcus aureus and Salmonella
Keywords: Onion, Coliform, Escherichia coli, Salmonella, Staphylococcus aureus.
Full paper in Arabic: PDF