Reem Alkhlif * (1) (2) , Mohammad Fawaz Azmeh (2), and Mahmoud abu Ghoura (1)
(1). Plant Protection department, Faculty of Agriculture, Damascus University, Damascus, Syria.
(2). National Commission for biotechnology, SYRIA
(*Corresponding author: Reem Alkhlif, Email reem.alkhlif@damascusuniversity.edu.sy )
Received: 7/07/2023 Accepted: 13/08/2023
Abstract:
The research was conducted in the greenhouse of Syrian National Commission for Biotechnology in Damascus during the 2021-2022 season, with the aim of studying the effect of Vesicular arbuscular mycorrhiza VAM Glomus spp. and Trichoderma harzianum fungi and bacterial isolates, which were isolated from the rhizosphere of Tomato plants and Trifolium purpureum, for controlling the effect of Fusarium solani that causes Tomato wilt, in planta. Bacterial isolates of Pseudomonas fluorescens and Bacillus cereus were identified according to several biochemical tests. Comparisons made between treatments were based on the dry weight of shoots and roots of treated and control plants, plant length, disease severity (%) according to the approved scale, and infection rate (%). The best isolates in controlling F.solani were the two isolates C33 and F63 related to Pseudomonas fluorescens with disease severity of 3.33 and 6.67% respectively, and increasing parameters of plants growth (plant height 70 and 63.25 cm, dry plant weight 5.05 and 4.75 g, dry root weight 4.35 and 4.9 g, respectively). In addition, the isolate K11 related to Bacillus cereus gave similar efficiency in disease control and growth parameters with a disease severity 6.67%, plant height 76 cm, dry plant weight 6.55 g, dry root weight 4.025 g. There were no significant differences between the results of the above mentioned parameters with plants treated with Trichoderma where results showed disease severity of 6.67%, plant height 69.75 cm, dry plant weight 6.05 g and dry root weight of 3.075 g. While, the isolate RIZN of B. cereus suppressed infection with the pathogen where disease severity became 3.33%, but it did not effect growth parameters. The greatest increase in dry weight of the roots was observed when plants were treated with the bacterial isolate F63, neither treatment with this isolate nor treatment with VAM revealed significant differences in dry weight when compared with the healthy control of the roots giving the following results 4.9, 4.4, and 3.25 g, respectively.
Keywords: Fusarium solani, Bacillus cereus, Pseudomonas fluorescens, Trichoderma harzianum, Vesicular arbuscular mycorrhiza, Syria.
Full paper in Arabic: pdf