Hala Al Zahr*(1), Fateh Khatib(1) and Naim Al Hussien(2)
(1). Plant Protection Department, Faculty of Agriculture, Aleppo University, Aleppo, Syria.
(2). Aleppo Research Center, General Commission of Scientific Agricultural Researches (GCSAR(, Aleppo, Syria.
(*Corresponding author: Hala Al-Zahr. E-Mail: halaalzaher.1990@gmail.com ).
Received: 11/ 12 /2021 Accepted: 15 /03 /2022
Abstract:
Dodder is a parasitic weed that attacks the aerial parts of plants, Crops, Trees, shrubs and weeds. The present research aimed to study the genetic diversity of dodder using Inter Simple Sequence Repeat (ISSR) technique, the samples collected from Aleppo, Hama and Homs, DNA was extracted from plant stems, its concentration, purity and quality were measured. Six ISSR primers were used to amplify the genomic DNA. The results showed the efficiency of the ISSR molecular markers in showing genetic diversity, where the percentage of polymorphism for all markers was 100%, and revealed the presence of large genetic diversity among the studied samples. The similarity ratio ranged between 0.04 – 0.89. The polymorphic information content varied among the markers, the best one was IS14, (PIC = 0.4). The studied samples were distributed in the dendogram into 4 clusters, according to the host.
Key words: Dodder, genetic diversity, molecular marker, ISSR.
Full paper in Arabic: pdf