Rusul Tawfiq Ali ¹ and Sajida A. Al-Shaheen¹

(1) Department of Animal Production, Faculty of Agriculture, University of Basra,Basra,Iraq.

 (*Corrsponding author: Rusul Tawfiq Ali.E-Mail: Rusul94119411@gmail.com).

Received: 17/10/2020                               Accepted: 01/11/2020

Abstract

This study was conducted with the aim of determining the association of the genetic polymorphisms of perilipin gene (PLIN1 gene) with body weight and abdominal fat weight of broiler chickens (Ross 308) using the nitrogen base sequence analysis technique. The results of DNA sequence analysis Conducted in the Korean company (Macrogen). showed the base change (T>C) at the 294 base pair position in the fifth exon region of the gene. The results of the genetic description showed the presence of two alleles (C, T) and two genotypes (CC, CT). The observed numbers of CC and CT genotypes were 9, 3 respectively. The allele C was of the highest allele frequency (0.875) and the allele T was of the lowest allele frequency (0.125). The CC genotype showed the highest genotype frequency (0.75%) and the CT were of the lowest genotype frequency (0.25%). The observed and expected allelic heterozygosity percentage was (0.25, 0.22), respectively. The average percentage of heterozygosity was (0.21). The results showed no significant (P ≥0.05) effect of the two genotypes; CC and CT of perilipin gene (PLIN1 gene) and gender on mean of body weight and abdominal weight at 5 and 7 weeks of age.

Key words: Hybrid Broiler Ross 308, Perilipin Gene PLIN1, Genotypic Polymorphism.

Full paper in Arabic: PDF