Sanaa K. Kadhim(1) and Jihad A. Ahmed*(1)

(1). Department of Pathology and Poultry diseases, Veterinary Medicine College, University of Basrah, Iraq.

(*Corrsponding author: Dr. Jihad Abdulameer Ahmed. E-Mail: jihad.ahmed@uobasrah.edu.iq).

Received: 22/9/2020 Accepted: 15/11/2020

Abstract

This study was conducted to find out the macroscopical and microscopical lesion as well as the pathological lesions, the hematological study, biochemical analysis, the histopathological study and histochemical study of liver abscesses in sheep. A regular visits were done to Al-Basrah abattoir during the period from October 2019 to January 2020 in which routine postmortem examination to slaughtered sheep was carried out. The livers from sheep showing pathological lesions or abnormalities were collected and thoroughly examined. The liver from 70 sheep were collected and thoroughly examined, sixty of them showed gross lesions (from left and right liver equally), other ten were normal in texture and morphology, used as a control. Our hematological results appear a significant (P≤0.05) increment in the total white blood cells counts (18.4±0.76) , lymphocytes counts (7.96±0.31) and neutrophils counts (8.11± 0.16) of ovine liver abscess group when compared to the control group which appear the total white blood cells counts, lymphocytes counts, and neutrophils counts were (11.16±0.47), (6.04±0.2) and (3.61±0.12) respectively. In addition, the results of the biochemical analysis of the study showed significant (P≤0.05) increase of Alanine aminotransferase (ALT) (48.5±5.4), Aspartate aminotransferase (AST) (32±1.9), C-Reactive Protein (CRP) (4.37±0.3) and lactate dehydrogenase (LDH) (195.3±1.19) of ovine liver abscess (OLA) group when compared to the control group were showed the ALT (19.0±0.5), AST (21.3±1.8), CRP (1.63±0.1) and LDH (169.3±14.8). The macroscopical results of our study of ovine liver abscess showed the plurality of these affected livers had a solo to multiple abscess formation; therefore, the grossly manifest on their surfaces which purulent material and a tender capsule with necrotic tissues in the central area. Besides, the gross examination revealed significant findings of necrotic cavity with a perceptible fibrous wall were observed in liver and multiple abscesses especially surrounded the biliary canal. The histopathological result of ovine liver abscess revealed a dilation of central vein filled with infiltration of inflammatory cells in the per-portal region, in addition, there are a present of a thick fibrous capsule formation surrounded abscess formation of the liver with inflammatory zone consist mainly polymorphnuclear cells surrounded the abscess capsule of the liver, also a present of large area of abscess formation in the liver parenchyma, which stained also positively in Mallory trichrome stain. The current study concluded that the ovine liver abscesses associated with dangerous increases in the cardinal hematological and biochemical biomarkers as an indicator to the harmful affection of the liver diseased with pyogenic infection.

Key words: Ovine liver abscess (OLA) , Biochemical, Hematology, Pathology.

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