Isolation of local Bacillus spp. Strains and Testing its Efficiency Against Wax Worm Galleria mellonella L. Larvae in Laboratory

Mohammad AL-Allan*(1) Nouraldin Daher-hjij(1) Nabeel Beig(1) Mahabba Ghannam(2) and Adel Almanoufi(2)

(1). Department of Entomology, Administration of Plant Protection Research, General Commission for Scientific Agricultural Research (GCSAR). Damascus, Syria.
(2). Department of Plant Diseases, Administration of Plant Protection Research, GCSAR., Damascus, Syria.

(*Corresponding author: Dr. Mohammad AL-Allan. Email:

Received: 05/06/2018                                               Accepted: 05/08/2018


This study was carried out to determine the efficiency of local Bacillus isolates to control large wax moth larvae (Galleria mellonella L.) at GCSAR laboratory in 2012. Infected larvae were collected from stored wax combs, and the bacteria Bacillus genus were isolated from dead larvae, or that showing disease symptoms of black brown spots on larvae cuticle. Bacterium was grown on T3 medium and identified according to biochemical tests, and the efficacy of isolates was determined on pure colonies of larvae. The results of biochemical tests showed that the isolates belong to Bacillus thuringiensis. Isolates were different in pathogenicity. Bt5 isolate was the most efficient to kill the larvae of large wax moth (72.4 %), and significantly superior all other isolates (p ≤ 0.01). Bt1 isolate showed a significant difference with control but non-significant difference with Bt2, Bt3, Bt4, Bt6 and Bt7 isolates.
Keywords: Bacillus thuringiensis, Entomopathogenic Bacteria, Wax worm, Local isolates.

Full paper in Arabic: PDF