Alaa Alshaal1, Khozama Kountar1, Rehab Almousa2*, and Shahinaz Abbas
1 Biotechnology Department,Laboratory and Quality Management General Commission for Agricultural Scientific Research, Syria.
(*Corresponding author: Rehab Almousa, Email:bebo_moussa13@yahoo.com, Mobile: +963938163029)
Received: 27/04 / 2025 Accepted: 14/12/ 2025
Abstract:
This research was conducted in the laboratories of the General Commission for Scientific Agricultural Research in Damascus/ Syria. Inter Simple Sequence Repeats (ISSR) technique was used to determine the genetic relationship among 81 genotypes (13 varieties and 68 lines) of durum wheat. All the 12 primers produced a total of 96 amplification bands and a polymorphic percentage of 96.26 %. The number of bands per primer varied from a minimum of 4 bands for the primers ISSR14, ISSR6 to a maximum of 18 bands for the primer ISSR7 with an average of 7.7 bands per primer. The average of polymorphic information content (PIC) was 0.337. The lowest distance (0.1111) was recorded between lines DW71 and DW77, this low value of the genetic distance indicates a great degree of genetic relationship. The largest distance (0.9722) was recorded between line DW74 and Bouhoth7, (0.9688) was recorded between line DW1 and the varieties Bouhoth5 and Cham7, indicating a far genetic relationship between them. It was noted from the genetic relationship tree that line DW81 was in a cluster by itself, while the other studied lines and varieties were fallen under the second cluster with varying degrees of genetic relationship. Our results indicate that the ISSR technique can be used as an important tool for characterization wheat genotypes, providing valuable information that helps breeders to select the most suitable varieties in plant breeding programs.
Keywords: Certificated Varieties and promising Lines, Durum wheat, Genetic relationship, ISSR technique.
Full paper in Arabic: PDF