Ahmed soufi^(1)*

(1). Department of Field Crops, Faculty of Agriculture, Tishreen University, Lattakia, Syria.

(*Corresponding author: Dr. Ahmed Soufi, E-mail: Ahmed soufi,  7mada.movo9@gmail.com, phone:00963991266905).

Received:  4/06/2024         Accepted:13/07/2024

Abstract: 

The research was conducted during the year 2024. The seeds were treated with three concentrations of Sodium azide (1, 3, 5 mM ) and with a soaking time of (6) hours.  In addition, to induce salinity stress, sodium chloride (NaCl) was used at concentrations (4, 8, 12 dS/m).  The experiment was implemented according to a randomized complete design (R.C.D.) in the village of Burj Islam – Latakia – Syria.  Three replicates for each treatment.  Some germination indicators of the treated seeds were measured (germination percentage (%)), morphological indicators of plants (plant height (cm/plant)), Morphophysiological indicators (total leaf surface area (cm2), )Net Photosynthesis Rate (mg/cm2/day)(, specific weight of leaves (g/cm2) and Leaf Area Index. High salinity concentration led to negative effects on all indicators studied. Treatment with the chemical mutagen NAN3 resulted in an increase in germination rate, plant height, total leaf surface area, net photosynthesis rate, leaf specific gravity and leaf area index. On the other side. Treatment with chemical mutagens under salinity stress conditions at low concentration (1 mM) outperformed all treatments and control. Therefore, we recommend soaking the seeds with a concentration of (1 mM) NAN3, due to its role in improving germination and the morphological and morphological characteristics of the local tobacco variety.

Keywords: soaking seeds, NAN3, tobacco plant, NaCl.

 Full paper in English: PDF