Evaluation of the total content of phenols and flavonoids and antioxidant activity of the methanolic extract of parsley (petroselinum crispum)using spectrophotometric methods.

Mohamad Fouti*(1), Ahmad Kamari(1), and Ahmad Ayoubi(2)

(1). Department of Animal Biology, Faculty of Science, University of Aleppo, Aleppo, Syria

(2). Dept of Pharmacognosy, Faculty of Pharmacy, University of Ebla, Idleb, Syria

(*Corresponding author: Mohamad Fouti: Mohammadfoti@gmail.com )

Received: 26/4/2025                       Accepted: 3/8/2025

Abstract: 

The parsley (Petroselinum crispum) has been known since ancient times for its nutritional and medicinal importance and its use as an anti-hyperglycemic and cardiovascular stimulant and digestive disorders, this research aimed to estimate the total content of phenols and flavonoids within the methanolic extract of the parsley plant (aerial parts only), and to evaluate the antioxidant efficacy based on the use of DPPH compound (2-2-biphenyl-1-becryl hydrazil) using spectroscopic methods. The results of the chemical survey showed the presence of many active groups in the methanolic extract of parsley such as phenols, flavonoids and alkaloids, and the phenols content was measured using the Foline Seocalto reagent and the flavonoid content was measured using aluminum chloride, the results showed that parsley contains a quantity of phenols amounting to (24.73 mg GAE/g DW) and flavonoids (mg GAE/g DW 16.22) and showed antioxidant activity of (IC50 = 73.45 mg/ml) From the previous results, we conclude the reason for the great effectiveness of the parsley plant in resisting many diseases and therefore it can be used to resist oxidative stress of cells because of its effective external antioxidants.

Keywords: Parsley, flavonoids, phenols, DPPH compound.

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