Testing the Efficacy of Wasal Flowers Extracts on Phytopathogenic Bacteria and Fungi

Zenib Suliman (1), Nwara Mohamed* (2) and Khaled El-Mir (1)

(1) Department of Horticulture, Faculty of Agriculture, Omar Mukhtar University, Al-Bayda, Libya.

(2) Department of plant protection, Faculty of Agriculture, Omar Mukhtar University, Al-Bayda, Libya.

(*Corresponding author: Nwara A. Mohamed, nwara.mohamed@omu.edu.ly).

Received: 23/11/2021                       Accepted: 22/12/2021

Abstract

This study aimed to evaluate the effectiveness of extracts of Spartium junceum L flowers on the growth of some plant pathogenic organisms, including the Bacteria (Agrobacterium tumefaciens, Xanthomonas campestris pv.vesicatoria and Erwinia cartovora& the Fungi Botrytis fabae, Fusarium oxysporum and Penicillium sp. . The flowers were collected from the area of Ras Al Hilal Green Mountain of Libya and ‎then dried, grinded, and extracted aqueously, alcoholically and with hexane and poisoned the medium PDA with a concentration of 5% of each extract separately in the presence of the control plates, while the bacteria were made by Disk Method with these extracts separately, and distributed on plates cultured with bacteria in the presence of the antibiotic. The results indicated a significant decrease in growth of the tested organisms under laboratory conditions by measuring the radial growth of the fungi colonies (cm) and displaying the inhibition zone for bacteria on the media. The results also recorded significant differences between the solvents used in the extraction. The alcoholic extract had a high effect on the growth of the fungi Botrytis fabae and Penicillium sp. and the growth of the bacteria Erwinia carotovora, while the aqueous extract affected the growth of Botrytis fabae, Agrobactrium and Erwinia, while the hexane extract affected the growth of the fungus Penicillium sp., we conclude from this study that it is possible to obtain compounds that inhibit the growth of some plant pathogenic organisms from extracts of Spartium junceum L flowers.‎

Key words:   Flowers, Aqueous, Methanolic, Hexane, Extracts phytopathogenic fungi, Phytopathogenic bacteria.

Full paper in Arabic: PDF