Manal Saleh*⁽¹⁾ Jamal Hajjar⁽²⁾ and Abdul kader Rahmo⁽¹⁾

(1). General Organization of Biotechnology, Damascus, Syria.
(2). Department of Arid Area Cultivation, King Faisal University, Saudi Arabia.
(3). Faculty of Agriculture, Damascus University, Damacus, Syria.

(*Corresponding author: Eng. Manal Saleh. E-Mail: manalcapno@gmail.com). 

Received: 10/05/ 2015                                     Accepted: 07/07/ 2015

Abstract

Chemical insecticides have a major role in insect pest control, especially organophosphates insecticides; their non-selective mode of action may cause devastating environmental problems. In vitro assays with cultured insect cells were used as the first step in toxicity studies and to achieve a simple, low cost and rapid system that allows testing of insecticides. In this study, the effect of the organophosphorus pesticide “Dimethoate” was evaluated in vitro on cultured Sf9 cell line.  Cell viability was estimated using Trypan blue stain. Analysis of regression was used to estimate the 50% inhibition of cell growth (IC₅₀), resulting in: IC₅₀ =75µM. Deformation of treated cells was observed, as cells became larger and included cavities; number of deformed cells was positively correlated with concentration of Dimethoate. This research presents an important example of applying in vitro insect cell culture as a useful tool in the prescreening of insecticides’ effect and mode of action, especially newly introduced ones.

Key words: Insect cell lines, In vitro test, Dimethoate, IC_50.

Full paper in Arabic:

 تقدير سمية مبيد دايمثوات باستخدام خط الخلايا الحشرية Sf9 المزروعة مخبرياً