Phytochemical Detection and Antimicrobial Activity of Centaurea ptosimopappa

Abdel Aleem Bello(1) and Tawfik Al-Boshi*(2)

(1). Plant Bilolgy Department, Faculty of Science, University of Aleppo, Aleppo, Syria.

(2). Depatment of Renewable Natural Resources and Ecology, Faculty of Agriculture, University of Aleppo, Aleppo, Syria.

(*Corresponding author: Dr. Tawfik Al-Boshi. E-Mail: dr.tawfik69@gmail.com).

Received: 20/02/2018                                Accepted: 11/04/2018

Abstract

The goal of the study was to detect the presence of active ingredients and the antimicrobial activity in the extracts of two different groups of Centaurea ptosimopappa Hayek, which are bald and furry, using thin layer chromatographic method and diffusion method within Agar well. This study was accomplished at Faculty of Science, Aleppo University. Phytochemical detection showed the presence of alkaloids, anthocyanins, tannins and steroidal triterpenes in the extracts of bald Centaurea ptosimopappa Hayek. While tannins, steroidal triterpenes, cardiac glycosides and flavonoids were detected in the extracts of furry group. Antimicrobial activities of these extracts were determined by Agar well diffusion method. The extracts of furry Centaurea ptosimopappa Hayek were clearly more active than bald Centaurea ptosimopappa Hayek. For instance, when we used 80 µl of the chloroform extract of furry plants the diameter of the inhibition zone was more than 20 mm for Staphylococcus aureus, but only 13 mm was noticed when 80 µl of the chloroform extract was used of bald plants.

Keywords: Phytochemical detection, Antimicrobial activity, Plant extracts, Centaurea ptosimopappa.

Full paper in Arabic: PDF