Catherine Nseir(1)*, Mohamad Housam Bahlawan(2), Ahmad Majar(3) , and Fadi Abbas(3)
(1) Crop Research Administration, General Commission for Scientific Agricultural Research, Damascus, Syria.
(2) Faculty of Agriculture, Aleppo University, Aleppo, Syria.
(3) General Commission for Scientific Agricultural Research, Damascus, Syria.
(*Corresponding author: Eng. Catherine Nseir, E-Mail: catynseir@gmail.com).
Received:12/09/2021 Accepted:21/11/2021
Abstract:
This experiment was conducted in the labs of General Commission for Scientific Agricultural Researches GCSAR, Homs Center, during 2020. PEG6000 was applied to the germination media with low osmotic potentials (OP) i.e. 0, -0.2, -0.6, -1.0, MPa, for screening 10 lentil genotypes. Complete randomized design CRD with three replicates was used. The germination %, germination speed, radicle and hypocotyl length, and the genotypes classified according to these attributes, relative drought stress index, and Z. distribution. The results exhibited a genetic difference between the genotypes concerning the response to the application of PEG6000 in the media. The germination percent was decreased with the decline of osmotic potential 0, -0.2, -0.6, -1.0, MPa, The percent of germination were (98.98, 97.22, 93.89, 50.56 %) respectively, as compared with the control. While the speed of germination were (7.45, 6.06, 5.25, 1.76) respectively. The length of epicotyl and radicle were estimated in all of tested osmotic potentials, the results showed that with decreasing the osmotic potential the length decreased, and the decrement in epicotyl was more than radicle. And seed vigor decreased with OP decreasing. According to the relation between germination percentage and speed through the z-distribution analysis, the genotypes were classified as tolerant to drought stress, i.e. G11, G15, G16, and. and could be sown successfully under the most drought conditions, compare to other genotypes.
Key words: Osmotic Potential, Polyethylene Glycol PEG6000, Germination Stage, Lentile.
Full paper in Arabic: pdf