Mamon Alamir*⁽¹⁾ and Samer Ebrahim ⁽¹⁾    

 (1). Microbiology Department, Veterinary Faculty- Hama University, Syria.

(*Corrsponding author: Dr. Mamon alamir. E-Mail: mamon.alamir@gmail.com)

Received: 15/01/2021                      Accepted:20/04/2021

Abstract                                                   

This research aims to investigate Necrotic Enteritis for infected birds through isolating and identification Clostridium perfringens. As well as detecting the existence of the Clostridium Perfringens from cecum for healthy birds. The study has also considered comparing the spread of Clostridium Perfringens in both healthy and infected flocks beside searching for Alpha Toxin Genes in isolates and studying the disease field condition. This has been achieved through collecting 246 samples from birds belonging to various herds and are common in different zones in Hama governorate throughout the period October 2018- October 2020. The results showed that Clostridium perfringens are existing in %22.64 of the samples taken from infected birds and in %5 of the samples taken from healthy ones. The PCR results showed the existence of alpha toxin gene in all the isolates that were collected from both infected and healthy birds. Male birds are more exposed to infection when compared to female ones as of necrotic enteritis. 82.61% of male birds have been recorded infected whereas 17.39% of female ones. The results showed that broilers were more infected than breeders, where it recorded 86.14% of broilers whereas 13.86% of breeders.

Keywords: Clostridium perfringens, necrotic enteritis, alpha toxin, chicken.

Full paper in Arabic: pdf