Eyad Dannoura (1)*, Hafez Mahfoud (1) and Ali Deeb (2)
(1). General Commission For Agricultural Scientific Research (GCSAR), Lattakia, Syria.
(2).Department Of Horticulture, Faculty, Of Agriculture, Tishreen University, Lattakia, Syria.
(*corresponding author: Dr. Eyad Dannoura. Email: eyed.dannoura@gmail.com )
Received: 10/08/2020 Accepted: 6/09/2020
Abstract
This study was carried out on the wild apple Malus trilobata (Lab) prevailing in Lattakia mountains, during (2013 – 2017), in ten areas (Pichraghe, Helbako, Almonaizlah, Raas al chaara, Aldalia, Aleriza, Beet zantot, Zanuo, Kherpat alsendiana, Gaopet bergal) , at an altitude between (730-1250) m above sea level. The study was achieved at Molecular Biotechnology lab/ Latakia Research Center. The aims of this study were to perform the molecular characterization by using RAPD technique. The results of the molecular characterization showed the efficiency of the primers used (16 primers) in detecting the genetic differences among the studied genotypes. Whereas all primers produced 92 fragments and showed efficiency in detecting genetic variation among the genotypes, only thirty-seven fragments were polymorphic and allowed to detect an average of genetic variability equal to 40.21% and helping in preparing the relevant dendrogram. The cluster analysis revealed that studied genotypes were divided into two groups with a variance ratio reached (29%). It also showed the existence of a large genetic base for the studied species, which allows it to be used in the breeding and genetic improvement programs for apples in Syria.
Keywords: Wild apple, Molecular characterization, Technique (RAPD), The cluster analysis.
Full paper in Arabic: PDF